Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   BT9727_RS25440 Genome accession   NC_005957
Coordinates   4917817..4919166 (-) Length   449 a.a.
NCBI ID   WP_000225367.1    Uniprot ID   Q6HB95
Organism   [Bacillus thuringiensis] serovar konkukian str. 97-27     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4912817..4924166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BT9727_RS25420 (BT9727_4868) secA 4913139..4915646 (-) 2508 WP_000579368.1 preprotein translocase subunit SecA -
  BT9727_RS25425 (BT9727_4869) hpf 4915923..4916465 (-) 543 WP_000671190.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BT9727_RS25430 (BT9727_4870) cspC 4916789..4916986 (-) 198 WP_001990088.1 cold shock protein CspC -
  BT9727_RS25435 (BT9727_4871) comFC 4917113..4917817 (-) 705 WP_011181921.1 comF operon protein ComFC -
  BT9727_RS25440 (BT9727_4872) comFA 4917817..4919166 (-) 1350 WP_000225367.1 ATP-dependent helicase ComFA Machinery gene
  BT9727_RS25445 (BT9727_4873) - 4919294..4920616 (-) 1323 WP_011181922.1 NlpC/P60 family protein -
  BT9727_RS25450 (BT9727_4874) - 4920870..4921184 (-) 315 WP_000400857.1 winged helix-turn-helix transcriptional regulator -
  BT9727_RS25455 (BT9727_4875) - 4921358..4922200 (-) 843 WP_000844766.1 DegV family protein -
  BT9727_RS25460 (BT9727_4876) - 4922440..4923075 (+) 636 WP_000926684.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51253.98 Da        Isoelectric Point: 9.7782

>NTDB_id=23387 BT9727_RS25440 WP_000225367.1 4917817..4919166(-) (comFA) [[Bacillus thuringiensis] serovar konkukian str. 97-27]
MVAGKQLLLEELSSDLRRELSDLKKKGEVICVQGVIKKVSKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELAPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=23387 BT9727_RS25440 WP_000225367.1 4917817..4919166(-) (comFA) [[Bacillus thuringiensis] serovar konkukian str. 97-27]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGTTTCTAAATATATATGTCAGCGCTGCGGGAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTAGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAAGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGCACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGAAGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(312-408)

Type III restriction enzyme, res subunit (ResIII)

(108-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6HB95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.153

93.096

0.486
  comFA Latilactobacillus sakei subsp. sakei 23K

40.75

89.087

0.363

Multiple sequence alignment