Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BT9727_RS20880 Genome accession   NC_005957
Coordinates   4049309..4050352 (-) Length   347 a.a.
NCBI ID   WP_001013193.1    Uniprot ID   -
Organism   [Bacillus thuringiensis] serovar konkukian str. 97-27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4044309..4055352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BT9727_RS20835 (BT9727_3976) - 4044736..4044936 (-) 201 WP_000106081.1 YqzE family protein -
  BT9727_RS20840 (BT9727_3977) aroK 4044975..4045472 (-) 498 WP_000836631.1 shikimate kinase AroK -
  BT9727_RS20845 (BT9727_3978) - 4045593..4046243 (-) 651 WP_000183880.1 2OG-Fe(II) oxygenase -
  BT9727_RS20850 (BT9727_3979) comGG 4046419..4046790 (-) 372 WP_001231191.1 competence type IV pilus minor pilin ComGG -
  BT9727_RS20855 (BT9727_3980) comGF 4046787..4047257 (-) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  BT9727_RS20860 (BT9727_3981) comGE 4047227..4047529 (-) 303 WP_000829460.1 competence type IV pilus minor pilin ComGE -
  BT9727_RS20865 (BT9727_3982) comGD 4047522..4047977 (-) 456 WP_000810393.1 comG operon protein ComGD -
  BT9727_RS20870 (BT9727_3983) comGC 4047974..4048273 (-) 300 WP_001178696.1 comG operon protein ComGC -
  BT9727_RS20875 (BT9727_3984) comGB 4048285..4049316 (-) 1032 WP_000472051.1 comG operon protein ComGB -
  BT9727_RS20880 (BT9727_3985) comGA 4049309..4050352 (-) 1044 WP_001013193.1 competence protein ComGA Machinery gene
  BT9727_RS20885 (BT9727_3986) - 4050558..4051253 (+) 696 WP_000434132.1 helix-turn-helix transcriptional regulator -
  BT9727_RS20890 (BT9727_3987) - 4051379..4051621 (+) 243 WP_000440720.1 DUF2626 domain-containing protein -
  BT9727_RS20895 (BT9727_3988) - 4051729..4053123 (+) 1395 WP_001094341.1 L-cystine transporter -
  BT9727_RS20900 (BT9727_3989) - 4053340..4053555 (-) 216 WP_001008320.1 DUF3912 family protein -
  BT9727_RS20905 (BT9727_3990) - 4053808..4054119 (+) 312 WP_001093243.1 hypothetical protein -
  BT9727_RS20910 (BT9727_3991) - 4054156..4054644 (-) 489 WP_000764247.1 hypothetical protein -
  BT9727_RS20915 (BT9727_3992) - 4054724..4055098 (-) 375 WP_001012359.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39377.92 Da        Isoelectric Point: 9.2320

>NTDB_id=23376 BT9727_RS20880 WP_001013193.1 4049309..4050352(-) (comGA) [[Bacillus thuringiensis] serovar konkukian str. 97-27]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=23376 BT9727_RS20880 WP_001013193.1 4049309..4050352(-) (comGA) [[Bacillus thuringiensis] serovar konkukian str. 97-27]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAGGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGGTTACTCGTATTTACTGGACCGACTGGTTCAGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

36.261

100

0.369

Multiple sequence alignment