Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BT9727_RS19645 Genome accession   NC_005957
Coordinates   3842112..3842546 (-) Length   144 a.a.
NCBI ID   WP_000811510.1    Uniprot ID   -
Organism   [Bacillus thuringiensis] serovar konkukian str. 97-27     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3837112..3847546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BT9727_RS19630 (BT9727_3743) - 3837877..3838434 (+) 558 WP_000062080.1 PadR family transcriptional regulator -
  BT9727_RS19635 (BT9727_3744) metE 3839361..3841649 (+) 2289 WP_001007629.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BT9727_RS19640 (BT9727_3745) comJ 3841686..3842075 (-) 390 WP_000424033.1 competence protein ComJ -
  BT9727_RS19645 (BT9727_3746) nucA/comI 3842112..3842546 (-) 435 WP_000811510.1 DNA-entry nuclease Machinery gene
  BT9727_RS19650 (BT9727_3747) - 3842783..3843532 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  BT9727_RS19655 (BT9727_3748) kinB 3843586..3844860 (-) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  BT9727_RS19660 (BT9727_3749) - 3845170..3845370 (+) 201 WP_000929281.1 hypothetical protein -
  BT9727_RS19665 (BT9727_3750) - 3845493..3846656 (+) 1164 WP_000434569.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16040.40 Da        Isoelectric Point: 8.4331

>NTDB_id=23373 BT9727_RS19645 WP_000811510.1 3842112..3842546(-) (nucA/comI) [[Bacillus thuringiensis] serovar konkukian str. 97-27]
MKQLKGIIISIIVILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=23373 BT9727_RS19645 WP_000811510.1 3842112..3842546(-) (nucA/comI) [[Bacillus thuringiensis] serovar konkukian str. 97-27]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGTAATTCTTTCTATTTTAGTAGCAGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment