Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   VP_RS01240 Genome accession   NC_004603
Coordinates   264267..266471 (-) Length   734 a.a.
NCBI ID   WP_005481427.1    Uniprot ID   Q87T17
Organism   Vibrio parahaemolyticus RIMD 2210633     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259267..271471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP_RS01220 (VP0249) hslU 260134..261465 (-) 1332 WP_005457198.1 HslU--HslV peptidase ATPase subunit -
  VP_RS01225 (VP0250) hslV 261489..262040 (-) 552 WP_005455873.1 ATP-dependent protease subunit HslV -
  VP_RS01230 (VP0251) ftsN 262205..262750 (-) 546 WP_005481405.1 cell division protein FtsN -
  VP_RS01235 (VP0252) cytR 262927..263973 (-) 1047 WP_254894148.1 DNA-binding transcriptional regulator CytR Regulator
  VP_RS01240 (VP0253) priA 264267..266471 (-) 2205 WP_005481427.1 primosomal protein N' Machinery gene
  VP_RS01245 (VP0255) rpmE 266766..266987 (+) 222 WP_005457203.1 50S ribosomal protein L31 -
  VP_RS01250 (VP0256) rpsJ 267445..267756 (+) 312 WP_004410492.1 30S ribosomal protein S10 -
  VP_RS01255 (VP0257) rplC 267771..268400 (+) 630 WP_005456132.1 50S ribosomal protein L3 -
  VP_RS01260 (VP0258) rplD 268418..269020 (+) 603 WP_005379556.1 50S ribosomal protein L4 -
  VP_RS01265 (VP0259) rplW 269017..269319 (+) 303 WP_004398471.1 50S ribosomal protein L23 -
  VP_RS01270 (VP0260) rplB 269335..270159 (+) 825 WP_005489461.1 50S ribosomal protein L2 -
  VP_RS01275 (VP0261) rpsS 270181..270459 (+) 279 WP_004394525.1 30S ribosomal protein S19 -
  VP_RS01280 (VP0262) rplV 270470..270802 (+) 333 WP_005383164.1 50S ribosomal protein L22 -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82271.83 Da        Isoelectric Point: 8.5714

>NTDB_id=23356 VP_RS01240 WP_005481427.1 264267..266471(-) (priA) [Vibrio parahaemolyticus RIMD 2210633]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLIWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAIKQQKVLQMLVNGALPHQEFVDQ
EITSTVLKSLEDKGWIERIEKKPAITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTSNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLIQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=23356 VP_RS01240 WP_005481427.1 264267..266471(-) (priA) [Vibrio parahaemolyticus RIMD 2210633]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGATTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGATCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAACCTCAACTGTACTAAAGTCTTTAGAAGATAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGCAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGCGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAACGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGATCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCCCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTTCTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCGCAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTGGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGAGATAGCACGCGCCGCAAAGGCAGTTTAGAAGATGCGCTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTGATAGGCACTCAAATGTTGGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTCGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAACACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCTCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAA
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)

DEAD/DEAH box helicase (DEAD)

(220-365)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

Helicase conserved C-terminal domain (Helicase_C)

(500-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87T17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.649

100

0.44

Multiple sequence alignment