Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CBI29_RS03050 Genome accession   NZ_CP021782
Coordinates   623868..624668 (+) Length   266 a.a.
NCBI ID   WP_005136899.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A85     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 618868..629668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBI29_RS03025 (CBI29_00574) - 619778..621076 (+) 1299 WP_001991191.1 glycosyltransferase family 4 protein -
  CBI29_RS03030 (CBI29_00575) - 621101..621652 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  CBI29_RS03035 (CBI29_00576) iscX 621752..621952 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  CBI29_RS03040 (CBI29_00577) ndk 622066..622497 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  CBI29_RS03045 (CBI29_00578) rlmN 622620..623852 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CBI29_RS03050 (CBI29_00579) pilF 623868..624668 (+) 801 WP_005136899.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CBI29_RS03055 (CBI29_00580) - 624659..625450 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  CBI29_RS03060 (CBI29_00581) ispG 625472..626587 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CBI29_RS03065 (CBI29_00582) hisS 626584..627876 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  CBI29_RS03070 (CBI29_00583) - 627905..628603 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30083.42 Da        Isoelectric Point: 9.3928

>NTDB_id=233501 CBI29_RS03050 WP_005136899.1 623868..624668(+) (pilF) [Acinetobacter baumannii strain A85]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=233501 CBI29_RS03050 WP_005136899.1 623868..624668(+) (pilF) [Acinetobacter baumannii strain A85]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment