Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6P73_RS14725 Genome accession   NZ_CP027387
Coordinates   2772324..2772983 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-3057     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2767324..2777983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P73_RS14700 parE 2767415..2769307 (+) 1893 WP_106875072.1 DNA topoisomerase IV subunit B -
  C6P73_RS14705 ygiN 2769355..2769669 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6P73_RS14710 mdaB 2769700..2770281 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6P73_RS14715 ygiZ 2770600..2770932 (+) 333 WP_000912120.1 DUF2645 family protein -
  C6P73_RS14720 qseC 2770978..2772327 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6P73_RS14725 ciaR 2772324..2772983 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6P73_RS14730 ygiW 2773135..2773527 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6P73_RS14735 ygiV 2773580..2774062 (+) 483 WP_000183500.1 GyrI-like domain-containing protein -
  C6P73_RS14745 parC 2774608..2776866 (+) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  C6P73_RS14750 plsC 2777099..2777836 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=233345 C6P73_RS14725 WP_001221502.1 2772324..2772983(-) (ciaR) [Escherichia coli strain 2014C-3057]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=233345 C6P73_RS14725 WP_001221502.1 2772324..2772983(-) (ciaR) [Escherichia coli strain 2014C-3057]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment