Detailed information NTG0233227

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: AYN27_RS04400
• Genome accession: NZ_CP027380
• Coordinates: 825879..826871 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain 2013C-3250
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 820879..831871

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AYN27_RS04370	ispD	820940..821650 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
AYN27_RS04375	ispF	821650..822129 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
AYN27_RS04380	truD	822126..823175 (+)	1050	WP_000568972.1	tRNA pseudouridine(13) synthase TruD	-
AYN27_RS04385	surE	823156..823917 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
AYN27_RS04390	pcm	823911..824537 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
AYN27_RS04395	nlpD	824722..825816 (+)	1095	WP_306365709.1	murein hydrolase activator NlpD	-
AYN27_RS04400	rpoS	825879..826871 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
AYN27_RS04405	-	826992..827399 (-)	408	WP_001208070.1	MarR family winged helix-turn-helix transcriptional regulator	-
AYN27_RS04410	-	827546..828139 (+)	594	WP_000767718.1	non-oxidative hydroxyarylic acid decarboxylases subunit B	-
AYN27_RS04415	-	828139..829560 (+)	1422	WP_000863199.1	non-oxidative hydroxyarylic acid decarboxylases subunit C	-
AYN27_RS04420	-	829571..829807 (+)	237	WP_000562984.1	non-oxidative hydroxyarylic acid decarboxylases subunit D	-
AYN27_RS04425	ygbN	829846..831210 (-)	1365	WP_000104439.1	GntP family transporter	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=233227 AYN27_RS04400 WP_000081550.1 825879..826871(+) (rpoS) [Escherichia coli strain 2013C-3250]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=233227 AYN27_RS04400 WP_000081550.1 825879..826871(+) (rpoS) [Escherichia coli strain 2013C-3250]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTTGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TATTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715