Detailed information NTG0232648

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: ND05_RS25350
• Genome accession: NZ_CP027351
• Coordinates: 4681877..4682869 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain 2014C-3655
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 4676877..4687869

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ND05_RS25325	ygbN	4677561..4678925 (+)	1365	WP_000104444.1	GntP family transporter	-
ND05_RS25330	-	4678935..4679171 (-)	237	WP_000562991.1	non-oxidative hydroxyarylic acid decarboxylases subunit D	-
ND05_RS25335	-	4679182..4680609 (-)	1428	WP_000863215.1	non-oxidative hydroxyarylic acid decarboxylases subunit C	-
ND05_RS25340	-	4680609..4681202 (-)	594	WP_000767718.1	non-oxidative hydroxyarylic acid decarboxylases subunit B	-
ND05_RS25345	-	4681349..4681756 (+)	408	WP_001208060.1	MarR family transcriptional regulator	-
ND05_RS25350	rpoS	4681877..4682869 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
ND05_RS25355	nlpD	4682932..4684026 (-)	1095	WP_306307908.1	murein hydrolase activator NlpD	-
ND05_RS25360	pcm	4684211..4684837 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
ND05_RS25365	surE	4684831..4685592 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
ND05_RS25370	truD	4685573..4686622 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
ND05_RS25375	ispF	4686619..4687098 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
ND05_RS25380	ispD	4687098..4687808 (-)	711	WP_000246142.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=232648 ND05_RS25350 WP_000081550.1 4681877..4682869(-) (rpoS) [Escherichia coli strain 2014C-3655]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=232648 ND05_RS25350 WP_000081550.1 4681877..4682869(-) (rpoS) [Escherichia coli strain 2014C-3655]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715