Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AZF34_RS17935 Genome accession   NZ_CP027347
Coordinates   3300413..3301072 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-4361     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3295413..3306072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZF34_RS17910 plsC 3295560..3296297 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  AZF34_RS17915 parC 3296530..3298788 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  AZF34_RS17925 ygiV 3299334..3299816 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  AZF34_RS17930 ygiW 3299869..3300261 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  AZF34_RS17935 ciaR 3300413..3301072 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  AZF34_RS17940 qseC 3301069..3302418 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  AZF34_RS17945 ygiZ 3302464..3302796 (-) 333 WP_000912120.1 DUF2645 family protein -
  AZF34_RS17950 mdaB 3303115..3303696 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  AZF34_RS17955 ygiN 3303727..3304041 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  AZF34_RS17960 parE 3304089..3305981 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=232545 AZF34_RS17935 WP_001221502.1 3300413..3301072(+) (ciaR) [Escherichia coli strain 2013C-4361]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=232545 AZF34_RS17935 WP_001221502.1 3300413..3301072(+) (ciaR) [Escherichia coli strain 2013C-4361]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment