Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VV1_RS06805 Genome accession   NC_004459
Coordinates   1421785..1423509 (+) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus CMCP6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1416785..1428509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV1_RS06780 (VV1_1434) gspM 1417189..1417839 (+) 651 WP_011079392.1 type II secretion system protein GspM -
  VV1_RS06785 (VV1_1435) - 1417832..1418152 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  VV1_RS06790 (VV1_1436) mshL 1418168..1419814 (+) 1647 WP_011079394.1 pilus (MSHA type) biogenesis protein MshL -
  VV1_RS06795 (VV1_1437) - 1419822..1420670 (+) 849 WP_011079395.1 AAA family ATPase -
  VV1_RS06800 (VV1_1438) - 1420667..1421788 (+) 1122 WP_043920937.1 tetratricopeptide repeat protein -
  VV1_RS06805 (VV1_1439) pilF 1421785..1423509 (+) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  VV1_RS06810 (VV1_1440) - 1423519..1424739 (+) 1221 WP_011079398.1 type II secretion system F family protein -
  VV1_RS06815 (VV1_1441) - 1424743..1425204 (+) 462 WP_039554781.1 hypothetical protein -
  VV1_RS06820 (VV1_1442) - 1425283..1425861 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VV1_RS25345 (VV1_1443) - 1425895..1426350 (+) 456 WP_011079401.1 type II secretion system protein -
  VV1_RS06830 (VV1_1444) - 1426403..1426888 (+) 486 WP_011079402.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VV1_RS06835 (VV1_1445) - 1426888..1427454 (+) 567 WP_011079403.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VV1_RS06840 (VV1_1446) - 1427454..1428143 (+) 690 WP_011079404.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=23248 VV1_RS06805 WP_011079397.1 1421785..1423509(+) (pilF) [Vibrio vulnificus CMCP6]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=23248 VV1_RS06805 WP_011079397.1 1421785..1423509(+) (pilF) [Vibrio vulnificus CMCP6]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGATCTTCTTGTAGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCGCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTCTACGTGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCTGCGGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAATTGGTGGAAGGCTTTGACCGCTATTATCGCCGTACCAAAGAGATTGCCAACTTTGCCGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATTGAAGATGACGACAGCGATGAAGTGACGGTTGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATTCAGGTGGGGGCGTCGGATATCCATATCGAACCAGACGCCAATGTGTTGCGTTTGCGCCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCACAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTT
ACTGGCCCTACGGGTTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGACATGGGGGCACCTGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAGACAGAAGATCATGTTGGCGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCAAATCAAGTG
GAAGCGGGTTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CAGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment