Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CCY86_RS14295 Genome accession   NZ_CP021652
Coordinates   3050575..3052074 (+) Length   499 a.a.
NCBI ID   WP_003262276.1    Uniprot ID   A0AAE3NGW1
Organism   Ralstonia solanacearum strain RS 488     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3045575..3057074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCY86_RS14270 (CCY86_14265) - 3046754..3047737 (+) 984 WP_014618022.1 KpsF/GutQ family sugar-phosphate isomerase -
  CCY86_RS14275 (CCY86_14270) - 3047770..3048357 (+) 588 WP_003262282.1 KdsC family phosphatase -
  CCY86_RS14280 (CCY86_14275) lptC 3048359..3048991 (+) 633 WP_003262280.1 LPS export ABC transporter periplasmic protein LptC -
  CCY86_RS14285 (CCY86_14280) lptA 3049059..3049655 (+) 597 WP_039555953.1 lipopolysaccharide transport periplasmic protein LptA -
  CCY86_RS14290 (CCY86_14285) lptB 3049689..3050477 (+) 789 WP_003262277.1 LPS export ABC transporter ATP-binding protein -
  CCY86_RS14295 (CCY86_14290) rpoN/rpoN1 3050575..3052074 (+) 1500 WP_003262276.1 RNA polymerase factor sigma-54 Machinery gene
  CCY86_RS14300 (CCY86_14295) hpf 3052126..3052479 (+) 354 WP_003262275.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CCY86_RS14305 (CCY86_14300) ptsN 3052830..3053285 (+) 456 WP_003262274.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CCY86_RS14310 (CCY86_14305) hprK 3053356..3054330 (+) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  CCY86_RS14315 (CCY86_14310) - 3054521..3054853 (+) 333 WP_039555955.1 PsiF family protein -
  CCY86_RS14320 (CCY86_14315) rapZ 3054947..3055837 (+) 891 WP_003262269.1 RNase adapter RapZ -
  CCY86_RS14325 (CCY86_14320) - 3055915..3056568 (+) 654 WP_003262267.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55158.49 Da        Isoelectric Point: 4.8522

>NTDB_id=232281 CCY86_RS14295 WP_003262276.1 3050575..3052074(+) (rpoN/rpoN1) [Ralstonia solanacearum strain RS 488]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPPPTE
SPPPAANGSDHADTAEASYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSTR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLESPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDESALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=232281 CCY86_RS14295 WP_003262276.1 3050575..3052074(+) (rpoN/rpoN1) [Ralstonia solanacearum strain RS 488]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGATGGTTCGGTCAGCGCGCTGCAGAGCAACAGCGCCACCCCGCCGCCGACCGAA
TCGCCACCGCCCGCCGCCAACGGCTCGGACCACGCCGACACAGCCGAAGCATCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCTCGCAAACCCCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCACGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAGGGCTACCTGACCGGCTCGCTCGACGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAACTCGACGAACTGCAGGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGTGCACGCTCGGCGGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGAGTCGCCAGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAGCTGCTCGCCGCGCGCGATTACACACGCCTGAAAAAAGCGCTGTCCGTCGACGAAAG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCGAACCTGCAGCAAAAGCTGCA
GGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment