Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LJ_RS02105 Genome accession   NC_005362
Coordinates   444839..446218 (+) Length   459 a.a.
NCBI ID   WP_004896725.1    Uniprot ID   Q74L28
Organism   Lactobacillus johnsonii NCC 533     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 439839..451218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJ_RS02085 (LJ0394) - 440291..441304 (-) 1014 WP_044496651.1 sugar transferase -
  LJ_RS02090 (LJ0395) pepC 441590..442939 (-) 1350 WP_004896722.1 aminopeptidase C -
  LJ_RS02095 (LJ0396) - 443136..444170 (+) 1035 WP_011161553.1 LCP family protein -
  LJ_RS02100 (LJ0397) - 444288..444839 (+) 552 WP_004895781.1 dUTP diphosphatase -
  LJ_RS02105 (LJ0398) radA 444839..446218 (+) 1380 WP_004896725.1 DNA repair protein RadA Machinery gene
  LJ_RS02110 (LJ0399) gltX 446300..447799 (+) 1500 WP_004895777.1 glutamate--tRNA ligase -
  LJ_RS02115 (LJ0400) cysS 447889..449301 (+) 1413 WP_011161554.1 cysteine--tRNA ligase -
  LJ_RS02120 (LJ0401) - 449294..449737 (+) 444 WP_004895772.1 Mini-ribonuclease 3 -
  LJ_RS02125 (LJ0402) rlmB 449724..450479 (+) 756 WP_004895769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50118.70 Da        Isoelectric Point: 7.6366

>NTDB_id=23176 LJ_RS02105 WP_004896725.1 444839..446218(+) (radA) [Lactobacillus johnsonii NCC 533]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=23176 LJ_RS02105 WP_004896725.1 444839..446218(+) (radA) [Lactobacillus johnsonii NCC 533]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAACTAAGGCAACTGCAAGCCGTCTGATGA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAGGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGCGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCCGCAAGTC
AAATAAAGATGCGGGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGATTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAACTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGCCATGTAACTAAAGAAGGAGCAATTGCTGGGCCTAAGATTATGGAACATATGGTTGATACCGTTCTTTAC
TTTGAAGGAGATGGGCACCACTCATATCGAATTTTAAGATCAGTAAAGAATCGTTTTGGTGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCAGATATCCAAGCTTTAGTAACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCGGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGCATGGCGGTTGCTTCAAGTTACAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTA
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCCGCAAAAGTAGGCTTTAAGCGAATTTTTAT
TCCTAAAAACAACTTAAACCAGGAGCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q74L28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.262

99.782

0.601
  radA Streptococcus pneumoniae Rx1

60.262

99.782

0.601
  radA Streptococcus pneumoniae D39

60.262

99.782

0.601
  radA Streptococcus pneumoniae R6

60.262

99.782

0.601
  radA Streptococcus pneumoniae TIGR4

60.262

99.782

0.601
  radA Streptococcus mitis SK321

60.262

99.782

0.601
  radA Bacillus subtilis subsp. subtilis str. 168

56.14

99.346

0.558

Multiple sequence alignment