Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   CBW60_RS03605 Genome accession   NZ_CP021491
Coordinates   741661..743454 (-) Length   597 a.a.
NCBI ID   WP_025267786.1    Uniprot ID   -
Organism   Leuconostoc mesenteroides strain WiKim33     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 736661..748454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBW60_RS03580 (CBW60_03570) pheS 738034..739083 (-) 1050 WP_025267790.1 phenylalanine--tRNA ligase subunit alpha -
  CBW60_RS03585 (CBW60_03575) - 739387..739755 (-) 369 WP_011679293.1 winged helix-turn-helix transcriptional regulator -
  CBW60_RS03590 (CBW60_03580) - 739775..740704 (-) 930 WP_025267789.1 phosphoglycerate dehydrogenase -
  CBW60_RS03595 (CBW60_03585) - 740704..741144 (-) 441 WP_025267788.1 nucleoside 2-deoxyribosyltransferase -
  CBW60_RS03600 (CBW60_03590) - 741161..741556 (-) 396 WP_025267787.1 cytidine deaminase -
  CBW60_RS03605 (CBW60_03595) pepF 741661..743454 (-) 1794 WP_025267786.1 oligoendopeptidase F Regulator
  CBW60_RS03610 (CBW60_03600) - 743512..744534 (-) 1023 WP_025267785.1 competence protein CoiA family protein -
  CBW60_RS03615 (CBW60_03605) - 744576..746021 (-) 1446 WP_011679289.1 ABC transporter substrate-binding protein/permease -
  CBW60_RS03620 (CBW60_03610) - 746088..746840 (-) 753 WP_025267784.1 amino acid ABC transporter ATP-binding protein -
  CBW60_RS03625 (CBW60_03615) - 746837..748279 (-) 1443 WP_011679287.1 ABC transporter substrate-binding protein/permease -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 67789.56 Da        Isoelectric Point: 4.6734

>NTDB_id=231002 CBW60_RS03605 WP_025267786.1 741661..743454(-) (pepF) [Leuconostoc mesenteroides strain WiKim33]
MSEKQLLRNQVPENLTWDLSSIFPTDEAWETAFNETRKSAQALSKYAGHVGDSADVLKEALEADLAVERQFEKVYVYAHQ
IYDQDTTNQKYSAYNARVQGLYAELSANTAFFQPEVLALSNEKLDAYLATAGLKKYAHFFDVLKARKPHTLPAEQEALLA
GASDIFNASEQTFGALDNSDIVFGDVHTETGEIVPLTNGLYSLLLESKDRSLREEAFTTLYDSYIALQNTFASTLSSHVK
GHNFLAKTRHYDSARQAAVTANDIPESVFETLTKTVDANLPLLHRFVSLRKKILNVDDVHSYDLYVPLVDEINFEVTYEK
AKDIVLAALAPLGEDYIAIVQKAFDERWIDVVENKGKRTGAYSSGSYDTKPFILLNWQNNLNNVYTLAHEMGHSVHSYFT
RHNQEYHYGDYPIFLAEIASTTNEGLLTDYLLKTNDDPKFQAYVLNQYLDGFKGTVFRQTQFAEFEQWIHEQSAAGVALT
ADEMSTFYGSLNQKFYGSDLFPDEAIAYEWARIPHFYYNFYVYQYSTGEAAATTLAERILTQNGAEDYKNYLKAGNSDYA
LNVIKKAGVNMSQPDYLNEAFKVFEERLNEFEKIMLK

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=231002 CBW60_RS03605 WP_025267786.1 741661..743454(-) (pepF) [Leuconostoc mesenteroides strain WiKim33]
ATGTCTGAAAAACAACTATTACGTAATCAAGTACCAGAAAATCTTACATGGGATCTATCCTCAATTTTTCCGACAGATGA
AGCTTGGGAAACCGCTTTTAATGAGACACGAAAGTCTGCACAAGCATTAAGCAAGTATGCTGGACACGTCGGGGATTCTG
CGGATGTTTTGAAAGAAGCCTTAGAAGCAGATTTAGCAGTAGAACGTCAATTCGAAAAAGTCTACGTATATGCTCATCAA
ATTTATGATCAAGATACAACTAATCAAAAGTATAGTGCATATAATGCTCGTGTTCAAGGCTTATATGCTGAATTAAGTGC
GAATACAGCCTTCTTCCAGCCTGAAGTGTTAGCATTGTCAAACGAGAAACTAGATGCATACTTAGCGACAGCTGGTTTGA
AAAAATATGCACACTTTTTTGACGTTTTAAAAGCTCGTAAACCGCATACGCTACCTGCAGAACAGGAAGCCTTACTAGCA
GGAGCAAGCGACATCTTTAATGCTTCTGAACAGACTTTTGGTGCATTGGATAATTCAGATATTGTTTTTGGTGATGTCCA
TACTGAAACAGGTGAAATTGTGCCATTGACAAATGGACTCTATTCATTGTTGTTGGAGTCTAAAGATCGTTCATTGCGTG
AGGAAGCGTTCACAACGCTTTATGATAGTTATATTGCTTTACAAAATACATTTGCCTCAACGTTATCTTCACATGTCAAG
GGACATAACTTTTTGGCTAAAACTCGTCACTATGATTCAGCTCGTCAAGCTGCTGTAACAGCCAATGATATTCCTGAGTC
AGTTTTTGAAACATTAACCAAAACAGTTGATGCCAATCTGCCATTACTACACCGTTTTGTATCATTACGTAAAAAAATTC
TTAATGTTGACGATGTACACTCTTACGATTTATATGTACCACTTGTTGATGAAATTAATTTTGAAGTAACATATGAAAAG
GCAAAAGACATTGTTTTGGCGGCTTTGGCACCGTTAGGCGAGGATTACATCGCTATTGTTCAAAAGGCTTTTGATGAACG
ATGGATTGATGTGGTTGAAAATAAGGGGAAGCGTACTGGCGCTTATTCAAGCGGTTCGTATGATACAAAACCATTCATTT
TGCTTAACTGGCAGAACAATTTGAATAATGTTTATACGTTGGCTCATGAAATGGGACATTCGGTTCATTCATACTTTACA
CGCCATAATCAAGAGTATCATTATGGTGATTATCCAATATTCTTAGCTGAAATTGCTTCAACTACTAATGAGGGATTATT
AACGGATTATTTGCTAAAGACAAATGATGATCCCAAGTTTCAGGCCTACGTATTGAACCAGTATCTAGATGGATTCAAAG
GAACAGTTTTCCGCCAAACTCAATTTGCTGAGTTTGAGCAATGGATTCATGAACAAAGTGCGGCTGGAGTGGCTTTGACA
GCTGATGAGATGAGTACATTCTATGGATCGTTGAATCAAAAGTTTTATGGATCGGACTTATTTCCAGATGAAGCTATTGC
TTATGAATGGGCACGTATTCCACATTTTTATTACAATTTCTATGTTTATCAATATTCGACAGGCGAAGCTGCTGCCACAA
CTTTGGCAGAACGCATACTAACACAAAATGGTGCTGAGGATTATAAGAACTATCTGAAAGCAGGTAATTCAGATTATGCT
TTAAATGTGATCAAAAAAGCTGGCGTTAACATGAGTCAACCGGACTATTTAAATGAAGCTTTCAAAGTGTTTGAAGAGCG
ATTAAATGAGTTTGAAAAAATCATGTTAAAGTGA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(204-582)

Oligopeptidase F (Peptidase_M3_N)

(115-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

52.614

99.33

0.523

Multiple sequence alignment