Detailed information NTG0230766

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BtSCAC15_RS06305
• Genome accession: NZ_CP021436
• Coordinates: 1208789..1212514 (+)
• Length: 1241 a.a.
• NCBI ID: WP_100654936.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain C15
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1203789..1217514

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BtSCAC15_RS06295 (BtSCAC15_06430)	-	1203802..1204980 (-)	1179	WP_127063857.1	tyrosine-type recombinase/integrase	-
BtSCAC15_RS06300 (BtSCAC15_06435)	addB	1205277..1208792 (+)	3516	WP_000058589.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BtSCAC15_RS06305 (BtSCAC15_06440)	addA	1208789..1212514 (+)	3726	WP_100654936.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BtSCAC15_RS06310 (BtSCAC15_06445)	-	1212527..1212814 (+)	288	WP_000255728.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
BtSCAC15_RS06315 (BtSCAC15_06450)	gerPF	1212952..1213167 (-)	216	WP_001141566.1	spore germination protein GerPF	-
BtSCAC15_RS06320 (BtSCAC15_06455)	-	1213210..1213596 (-)	387	WP_000902324.1	spore germination protein GerPE	-
BtSCAC15_RS06325 (BtSCAC15_06460)	gerPD	1213612..1213806 (-)	195	WP_001052807.1	spore germination protein GerPD	-
BtSCAC15_RS06330 (BtSCAC15_06465)	gerPC	1213813..1214427 (-)	615	WP_001070760.1	spore germination protein GerPC	-
BtSCAC15_RS06335 (BtSCAC15_06470)	gerPB	1214495..1214701 (-)	207	WP_001012508.1	spore germination protein GerPB	-
BtSCAC15_RS06340 (BtSCAC15_06475)	gerPA	1214716..1214937 (-)	222	WP_001111187.1	spore germination protein GerPA	-
BtSCAC15_RS06345 (BtSCAC15_06480)	-	1215034..1215213 (-)	180	WP_000462841.1	aspartyl-phosphate phosphatase Spo0E family protein	-
BtSCAC15_RS06350 (BtSCAC15_06485)	-	1215461..1216360 (+)	900	WP_001213071.1	fumarylacetoacetate hydrolase family protein	-
BtSCAC15_RS06355 (BtSCAC15_06490)	-	1216397..1216678 (-)	282	WP_000926857.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142731.90 Da
• Isoelectric Point: 4.8010

>NTDB_id=230766 BtSCAC15_RS06305 WP_100654936.1 1208789..1212514(+) (addA) [Bacillus thuringiensis strain C15]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVALLGTLSSAARESWTSLYEAMQNVSWQTLKRIKKSDYNEEVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLSDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=230766 BtSCAC15_RS06305 WP_100654936.1 1208789..1212514(+) (addA) [Bacillus thuringiensis strain C15]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCTAACGGACG
TGATATTTTAGTTGCTGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGCATCGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAGGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGAGGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAGGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGACACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AGCCAGGCTTATTCCTAGGAAAGTATAAACGTTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGCTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCAGCAGAATTATTATGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGTCCGGTGCAATA
TCGCGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATACGCTGACCTCGCGACTGGTTATTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCCGCTGTACTTCGTTCCCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGACAAGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCCCTAGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACAACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGC
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCCGGGGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGATATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACACTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTTCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGATCAAGCGAAACCAATTTTA
GAGGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTGGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535