Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BtSCAC15_RS00505 Genome accession   NZ_CP021436
Coordinates   95199..96575 (+) Length   458 a.a.
NCBI ID   WP_001085202.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain C15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 90199..101575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BtSCAC15_RS00485 (BtSCAC15_00485) ctsR 90396..90857 (+) 462 WP_001244565.1 transcriptional regulator CtsR -
  BtSCAC15_RS00490 (BtSCAC15_00490) - 91028..91576 (+) 549 WP_000128400.1 UvrB/UvrC motif-containing protein -
  BtSCAC15_RS00495 (BtSCAC15_00495) - 91581..92645 (+) 1065 WP_000050843.1 protein arginine kinase -
  BtSCAC15_RS00500 (BtSCAC15_00500) clpC 92668..95103 (+) 2436 WP_000971183.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BtSCAC15_RS00505 (BtSCAC15_00505) radA 95199..96575 (+) 1377 WP_001085202.1 DNA repair protein RadA Machinery gene
  BtSCAC15_RS00510 (BtSCAC15_00510) disA 96579..97652 (+) 1074 WP_127063737.1 DNA integrity scanning diadenylate cyclase DisA -
  BtSCAC15_RS00515 (BtSCAC15_00515) - 97813..98922 (+) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  BtSCAC15_RS00520 (BtSCAC15_00520) ispD 98939..99619 (+) 681 WP_000288284.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BtSCAC15_RS00525 (BtSCAC15_00525) ispF 99736..100212 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49844.48 Da        Isoelectric Point: 7.1422

>NTDB_id=230759 BtSCAC15_RS00505 WP_001085202.1 95199..96575(+) (radA) [Bacillus thuringiensis strain C15]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=230759 BtSCAC15_RS00505 WP_001085202.1 95199..96575(+) (radA) [Bacillus thuringiensis strain C15]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCCGTTGTATCATCTAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAGGTGACATCAGC
TCCTGGTAGTGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTCA
TCGTAGGACATGTGACAAAAGAAGGTGCAATTGCGGGGCCGCGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGACGAACCGGCAATTGATTTAGCTG
TCGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGATGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTGGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment