Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CBR65_RS14975 Genome accession   NZ_CP021382
Coordinates   3614510..3615598 (-) Length   362 a.a.
NCBI ID   WP_087467604.1    Uniprot ID   -
Organism   Cellvibrio sp. PSBB006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3591700..3627019 3614510..3615598 within 0


Gene organization within MGE regions


Location: 3591700..3627019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBR65_RS14865 (CBR65_14845) - 3591745..3592512 (+) 768 WP_157672092.1 type II secretion system protein N -
  CBR65_RS14870 (CBR65_14850) - 3592880..3594583 (+) 1704 WP_232461206.1 glycosyl hydrolase family 18 protein -
  CBR65_RS14875 (CBR65_14855) - 3594672..3595754 (-) 1083 WP_198300751.1 glycoside hydrolase family 16 protein -
  CBR65_RS14885 (CBR65_14865) - 3595861..3597282 (+) 1422 WP_087467593.1 polysaccharide lyase -
  CBR65_RS14890 (CBR65_14870) - 3597408..3598412 (+) 1005 WP_369825622.1 FAD:protein FMN transferase -
  CBR65_RS14895 (CBR65_14875) - 3598754..3599206 (+) 453 WP_087469090.1 AraC family transcriptional regulator -
  CBR65_RS14900 (CBR65_14880) - 3599194..3601200 (+) 2007 WP_232461207.1 tetratricopeptide repeat protein -
  CBR65_RS14905 (CBR65_14885) - 3601197..3604097 (+) 2901 WP_232461208.1 tetratricopeptide repeat protein -
  CBR65_RS14910 (CBR65_14890) - 3604084..3604755 (+) 672 WP_087467594.1 tetratricopeptide repeat protein -
  CBR65_RS14915 (CBR65_14895) - 3604759..3605112 (+) 354 WP_087467595.1 hypothetical protein -
  CBR65_RS14920 (CBR65_14900) - 3605239..3605913 (+) 675 WP_087467596.1 MotA/TolQ/ExbB proton channel family protein -
  CBR65_RS14925 (CBR65_14905) - 3605988..3606527 (+) 540 WP_087467597.1 biopolymer transporter ExbD -
  CBR65_RS14930 (CBR65_14910) - 3606524..3607048 (+) 525 WP_087467598.1 biopolymer transporter ExbD -
  CBR65_RS14935 (CBR65_14915) - 3607048..3608004 (+) 957 WP_087467599.1 AgmX/PglI C-terminal domain-containing protein -
  CBR65_RS14940 (CBR65_14920) - 3608063..3609115 (-) 1053 WP_087467600.1 OmpA family protein -
  CBR65_RS14945 (CBR65_14925) - 3609335..3610384 (-) 1050 WP_087467601.1 OmpA family protein -
  CBR65_RS14950 (CBR65_14930) trmB 3610560..3611291 (-) 732 WP_232461209.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  CBR65_RS14955 (CBR65_14935) - 3611334..3611711 (-) 378 WP_087469094.1 DUF423 domain-containing protein -
  CBR65_RS14960 (CBR65_14940) rpoH 3611781..3612635 (-) 855 WP_087467602.1 RNA polymerase sigma factor RpoH -
  CBR65_RS14965 (CBR65_14945) ftsX 3612829..3613848 (-) 1020 WP_232461210.1 permease-like cell division protein FtsX -
  CBR65_RS14970 (CBR65_14950) ftsE 3613845..3614513 (-) 669 WP_087467603.1 cell division ATP-binding protein FtsE -
  CBR65_RS14975 (CBR65_14955) pilA 3614510..3615598 (-) 1089 WP_087467604.1 signal recognition particle-docking protein FtsY Machinery gene
  CBR65_RS14980 (CBR65_14960) rsmD 3615742..3616293 (+) 552 WP_369825623.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CBR65_RS14985 (CBR65_14965) - 3616295..3616723 (-) 429 WP_087467606.1 fibronectin type III domain-containing protein -
  CBR65_RS14990 (CBR65_14970) coaD 3616976..3617458 (+) 483 WP_087467607.1 pantetheine-phosphate adenylyltransferase -
  CBR65_RS14995 (CBR65_14975) mutM 3617481..3618293 (-) 813 WP_087467608.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CBR65_RS15000 (CBR65_14980) - 3618572..3619972 (-) 1401 WP_087467609.1 HDOD domain-containing protein -
  CBR65_RS15005 (CBR65_14985) - 3620077..3621741 (-) 1665 WP_087467610.1 cation:proton antiporter -
  CBR65_RS15010 (CBR65_14990) recQ 3621817..3623616 (-) 1800 WP_087467611.1 DNA helicase RecQ -
  CBR65_RS15015 (CBR65_14995) - 3623654..3624961 (-) 1308 WP_087467612.1 dicarboxylate/amino acid:cation symporter -
  CBR65_RS15020 (CBR65_15000) - 3625000..3625278 (-) 279 WP_087467613.1 HU family DNA-binding protein -
  CBR65_RS15025 (CBR65_15005) - 3625478..3626071 (+) 594 WP_087467614.1 chorismate lyase -
  CBR65_RS15030 (CBR65_15010) ubiA 3626093..3627019 (+) 927 WP_087467615.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 38925.41 Da        Isoelectric Point: 4.8424

>NTDB_id=230329 CBR65_RS14975 WP_087467604.1 3614510..3615598(-) (pilA) [Cellvibrio sp. PSBB006]
MFFKKLFNKSEPESPEAPEVTQEQTPAEPSGEEVSAEPPLLAETPAQPEPAAKPGFFARIKQGLSRTSNNFAEGLGNLFL
GRKNIDDDLLEDIESQLLIADVGIEATTEIVDNLTQRVARKQLADADALFRALRELLAGLLRPVEQPLVIDKTRQPYVIL
VVGVNGVGKTTTIGKLAKRLQNEGNKVMLAAGDTFRAAAVEQLQVWGQRNNVPVIAQHTGADSASVIFDAVQAAQSRKID
VIIADTAGRLHNKDHLMDELSKVKRVMAKLDVSAPHEVLLVLDAGTGQNAVNQAQQFIDAAGVTGIALTKLDGTAKGGVI
FALSKKFGLPVRFIGVGEGIDDLQPFAAEPFIQALFNQQDSL

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=230329 CBR65_RS14975 WP_087467604.1 3614510..3615598(-) (pilA) [Cellvibrio sp. PSBB006]
ATGTTCTTCAAGAAGCTCTTCAACAAGTCTGAACCCGAGTCTCCGGAGGCTCCCGAGGTTACCCAGGAACAAACGCCTGC
GGAGCCATCCGGGGAGGAAGTTTCCGCTGAGCCCCCGCTCCTGGCAGAAACGCCCGCCCAGCCGGAGCCCGCTGCCAAAC
CCGGTTTTTTTGCGCGGATCAAACAAGGCCTGAGTCGCACCAGCAATAACTTCGCCGAAGGGCTGGGCAACCTGTTCCTC
GGCAGAAAAAATATCGACGATGACTTGCTGGAGGATATCGAAAGCCAACTGTTGATCGCCGATGTGGGTATTGAAGCAAC
CACCGAGATTGTCGATAACCTCACCCAGCGCGTCGCCCGCAAGCAATTGGCGGATGCCGATGCCTTGTTTCGCGCGTTAC
GTGAATTGCTCGCTGGATTGCTACGTCCGGTGGAACAGCCGCTGGTCATTGATAAAACCAGACAGCCTTACGTCATTTTG
GTCGTCGGGGTAAACGGCGTGGGCAAGACCACCACCATCGGCAAACTGGCCAAGCGCCTGCAAAACGAAGGCAATAAAGT
CATGTTGGCGGCGGGCGATACCTTCCGTGCTGCTGCGGTGGAACAGCTACAGGTCTGGGGTCAGCGCAACAATGTGCCGG
TTATCGCCCAGCATACCGGTGCAGATAGTGCATCGGTCATCTTCGATGCCGTGCAAGCTGCTCAATCGCGCAAGATAGAT
GTGATCATCGCCGATACCGCCGGGCGCCTACACAACAAGGATCATCTGATGGATGAGCTGAGCAAGGTCAAGCGCGTAAT
GGCCAAGCTGGATGTCAGCGCACCCCATGAAGTTCTGCTGGTATTGGATGCCGGAACCGGTCAAAACGCCGTCAATCAAG
CACAACAGTTTATCGATGCCGCCGGCGTGACCGGTATCGCCTTGACCAAACTGGATGGCACCGCCAAAGGCGGCGTGATC
TTCGCGCTGAGCAAGAAATTCGGCCTGCCGGTGCGCTTTATCGGCGTGGGCGAGGGTATCGATGACCTGCAGCCCTTTGC
GGCCGAGCCTTTTATCCAGGCGCTGTTTAATCAACAGGACAGCCTGTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(157-356)

SRP54-type protein, helical bundle domain (SRP54_N)

(72-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.585

95.856

0.494

Multiple sequence alignment