Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CBP33_RS17450 Genome accession   NZ_CP021359
Coordinates   3718842..3720431 (-) Length   529 a.a.
NCBI ID   WP_086913635.1    Uniprot ID   A0A240U5T0
Organism   Acidovorax carolinensis strain NA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3713842..3725431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP33_RS17430 (CBP33_17425) - 3715559..3716398 (-) 840 WP_086913631.1 UPF0149 family protein -
  CBP33_RS17435 (CBP33_17430) - 3716492..3717034 (-) 543 WP_086913632.1 putative toxin-antitoxin system toxin component, PIN family -
  CBP33_RS17440 (CBP33_17435) - 3717024..3718316 (-) 1293 WP_086913633.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CBP33_RS17445 (CBP33_17440) - 3718340..3718771 (-) 432 WP_086913634.1 acyl-CoA thioesterase -
  CBP33_RS17450 (CBP33_17445) rpoN/rpoN1 3718842..3720431 (-) 1590 WP_086913635.1 RNA polymerase factor sigma-54 Machinery gene
  CBP33_RS17455 (CBP33_17450) lptB 3720431..3721204 (-) 774 WP_236748716.1 LPS export ABC transporter ATP-binding protein -
  CBP33_RS17460 (CBP33_17455) lptA 3721216..3721869 (-) 654 WP_086913637.1 lipopolysaccharide transport periplasmic protein LptA -
  CBP33_RS17465 (CBP33_17460) - 3721969..3722625 (-) 657 WP_086913638.1 thiol:disulfide interchange protein DsbA/DsbL -
  CBP33_RS17470 (CBP33_17465) - 3722710..3723360 (-) 651 WP_086913639.1 SPOR domain-containing protein -
  CBP33_RS17475 (CBP33_17470) argS 3723373..3725082 (-) 1710 WP_086913640.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58236.34 Da        Isoelectric Point: 4.7451

>NTDB_id=230165 CBP33_RS17450 WP_086913635.1 3718842..3720431(-) (rpoN/rpoN1) [Acidovorax carolinensis strain NA2]
MKPGLSLRVSQHLSLTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAPREEFGLEAADTPAQADDYSADDAMY
SGAAGASSTSAEGQNDAETPSASADEPDWSGDGTVEMAPNDAEWGGDAPARNRNDSDGDEADATELARSHESLTSFLHRQ
ALALRLSEIDRAALRFLIESLNDDGYLEEPLEELATGLAGEGNLEQIDELVHRFTVAQRLLQSLEPVGVGARNLAECLTL
QLTALAADENSAIDPDTVQTALRICQQPLEMLARRDIRRLTQLCGDGEERTRAAMALIARLEPRPGRRFADVERNIIVPD
VIVRKAGRANGRDGQHNFIVQLNPDVMPRLRVHDIYAGALRGHKGGEGHQGMQQRLQEARWFIKNIQQRFDTILRVSRAI
VERQKNFFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPIGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VAAENPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=230165 CBP33_RS17450 WP_086913635.1 3718842..3720431(-) (rpoN/rpoN1) [Acidovorax carolinensis strain NA2]
ATGAAACCGGGTCTTTCGCTGCGCGTTTCGCAGCACCTGTCGCTCACGCCGCAGTTGCAGCAATCCATCCGGCTGTTGCA
GCTGTCGACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGATGAAAACCCGTTTCTGGAGCGCACCGCCGAAGAAG
CGCCGCGCGAGGAATTTGGCCTGGAGGCGGCCGACACCCCCGCGCAGGCCGACGACTACAGCGCCGATGACGCTATGTAT
TCAGGAGCTGCTGGCGCAAGCAGTACAAGCGCTGAGGGCCAAAATGATGCTGAAACCCCCAGCGCCAGCGCGGATGAGCC
CGACTGGAGCGGCGACGGCACCGTGGAAATGGCGCCCAACGATGCCGAGTGGGGTGGCGATGCACCCGCCCGCAACCGCA
ACGACTCCGACGGCGACGAGGCCGATGCCACCGAGCTGGCGCGCTCGCACGAGTCGCTCACGTCCTTTCTGCACCGTCAG
GCCCTGGCGCTGCGCCTGTCCGAGATCGACCGCGCCGCGCTGCGTTTTCTGATCGAATCGCTGAACGACGATGGCTACCT
CGAAGAACCACTTGAAGAGCTGGCCACGGGCCTGGCCGGCGAGGGCAACCTGGAGCAGATCGACGAGCTGGTGCACCGTT
TCACCGTGGCGCAACGCCTGCTGCAAAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCAACCTGGCCGAATGCCTGACGCTG
CAGCTGACCGCCCTGGCCGCCGATGAAAACAGCGCGATCGACCCCGACACCGTGCAGACCGCTCTGCGCATCTGCCAGCA
GCCGCTGGAGATGCTGGCGCGCCGCGACATCCGCCGCCTGACGCAGCTGTGTGGCGATGGCGAGGAGCGCACCCGCGCCG
CCATGGCGCTGATTGCCCGCCTGGAGCCGCGCCCCGGCCGCCGCTTTGCCGATGTCGAGCGCAACATCATCGTGCCCGAC
GTCATCGTGCGCAAGGCGGGCCGTGCCAACGGCCGCGATGGCCAGCACAACTTCATCGTTCAGCTCAATCCCGACGTGAT
GCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCTCTGCGCGGTCACAAGGGCGGCGAGGGCCACCAGGGCATGCAGC
AGCGCCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTTGACACCATCCTGCGCGTCTCCCGCGCCATC
GTCGAGCGGCAGAAGAATTTTTTCACCCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGACATCGCCGACGAGCT
GGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCGATCGGTACCTATGAGCTCAAAT
ACTTCTTTGGCTCGGGCCTGGGCACCGAGACCGGCGGCAATGCCTCCAGCACGGCGGTGCGTGCGCTGATCAAGCAGTTC
GTCGCCGCTGAAAACCCCGCCAAGCCGCTGTCCGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACGGTGGCCAAGTACCGCGAGGCGCTCAAGATCGCGCCCGCCAACCTGCGCAAGGCGCTCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-356)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240U5T0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.084

100

0.537

Multiple sequence alignment