Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   C5099_RS23915 Genome accession   NZ_CP027103
Coordinates   4599315..4599983 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain RM14723     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4594315..4604983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5099_RS23885 (C5099_23875) rfaQ 4594493..4595551 (-) 1059 WP_000360417.1 lipopolysaccharide core heptosyltransferase RfaQ -
  C5099_RS23890 (C5099_23880) waaA 4595964..4597241 (+) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  C5099_RS23895 (C5099_23885) coaD 4597249..4597728 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  C5099_RS23900 (C5099_23890) mutM 4597767..4598576 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  C5099_RS23905 (C5099_23895) rpmG 4598674..4598841 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  C5099_RS23910 (C5099_23900) rpmB 4598862..4599098 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  C5099_RS23915 (C5099_23905) radC 4599315..4599983 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  C5099_RS23920 (C5099_23910) coaBC 4600155..4601375 (+) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  C5099_RS23925 (C5099_23915) dut 4601353..4601811 (+) 459 WP_000976070.1 dUTP diphosphatase -
  C5099_RS23930 (C5099_23920) slmA 4601918..4602514 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  C5099_RS23935 (C5099_23925) pyrE 4602551..4603192 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  C5099_RS23940 (C5099_23930) rph 4603258..4603974 (-) 717 WP_001247093.1 ribonuclease PH -
  C5099_RS23950 (C5099_23940) yicC 4604101..4604964 (+) 864 WP_000621332.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=230077 C5099_RS23915 WP_001297375.1 4599315..4599983(-) (radC) [Escherichia coli strain RM14723]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=230077 C5099_RS23915 WP_001297375.1 4599315..4599983(-) (radC) [Escherichia coli strain RM14723]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment