Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C5099_RS21555 Genome accession   NZ_CP027103
Coordinates   4151656..4153089 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain RM14723     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4146656..4158089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5099_RS21525 (C5099_21515) yrbG 4147214..4148191 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  C5099_RS21530 (C5099_21520) kdsD 4148205..4149191 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  C5099_RS21535 (C5099_21525) kdsC 4149212..4149778 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  C5099_RS21540 (C5099_21530) lptC 4149775..4150350 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  C5099_RS21545 (C5099_21535) lptA 4150319..4150876 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  C5099_RS21550 (C5099_21540) lptB 4150883..4151608 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  C5099_RS21555 (C5099_21545) rpoN/rpoN1 4151656..4153089 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  C5099_RS21560 (C5099_21550) hpf 4153112..4153399 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  C5099_RS21565 (C5099_21555) ptsN 4153517..4154008 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  C5099_RS21570 (C5099_21560) rapZ 4154054..4154908 (+) 855 WP_000243741.1 RNase adapter RapZ -
  C5099_RS21575 (C5099_21565) npr 4154905..4155177 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  C5099_RS21580 (C5099_21570) yrbL 4155391..4156023 (+) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  C5099_RS21585 (C5099_21575) mtgA 4156020..4156748 (-) 729 WP_001357723.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  C5099_RS21590 (C5099_21580) elbB 4156745..4157398 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=230068 C5099_RS21555 WP_000809051.1 4151656..4153089(+) (rpoN/rpoN1) [Escherichia coli strain RM14723]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=230068 C5099_RS21555 WP_000809051.1 4151656..4153089(+) (rpoN/rpoN1) [Escherichia coli strain RM14723]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGCCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CTCGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment