Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AB895_RS09955 Genome accession   NZ_CP021342
Coordinates   2072521..2073321 (+) Length   266 a.a.
NCBI ID   WP_188198396.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain B8342     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2067521..2078321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB895_RS09930 (AB895_2019) - 2068431..2069729 (+) 1299 WP_000348499.1 glycosyltransferase family 1 protein -
  AB895_RS09935 (AB895_2020) - 2069754..2070305 (+) 552 WP_000669564.1 phosphatase PAP2 family protein -
  AB895_RS09940 (AB895_2021) iscX 2070405..2070605 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  AB895_RS09945 (AB895_2022) ndk 2070719..2071150 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  AB895_RS09950 (AB895_2023) rlmN 2071273..2072505 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AB895_RS09955 (AB895_2024) pilF 2072521..2073321 (+) 801 WP_188198396.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AB895_RS09960 (AB895_2025) - 2073312..2074103 (+) 792 WP_000405161.1 RodZ domain-containing protein -
  AB895_RS09965 (AB895_2026) ispG 2074125..2075240 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AB895_RS09970 (AB895_2027) hisS 2075237..2076529 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  AB895_RS09975 (AB895_2028) - 2076558..2077256 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30066.37 Da        Isoelectric Point: 9.2307

>NTDB_id=230063 AB895_RS09955 WP_188198396.1 2072521..2073321(+) (pilF) [Acinetobacter baumannii strain B8342]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQIERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=230063 AB895_RS09955 WP_188198396.1 2072521..2073321(+) (pilF) [Acinetobacter baumannii strain B8342]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATAGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.462

97.744

0.718

Multiple sequence alignment