Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FORC77_RS02020 Genome accession   NZ_CP027030
Coordinates   422238..423962 (+) Length   574 a.a.
NCBI ID   WP_131069623.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_077     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417238..428962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC77_RS01995 (FORC77_0360) gspM 417642..418292 (+) 651 WP_011079392.1 type II secretion system protein GspM -
  FORC77_RS02000 (FORC77_0361) - 418285..418605 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  FORC77_RS02005 (FORC77_0362) mshL 418621..420267 (+) 1647 WP_072615906.1 pilus (MSHA type) biogenesis protein MshL -
  FORC77_RS02010 (FORC77_0363) - 420275..421123 (+) 849 WP_065090056.1 AAA family ATPase -
  FORC77_RS02015 (FORC77_0364) - 421120..422241 (+) 1122 WP_131069622.1 tetratricopeptide repeat protein -
  FORC77_RS02020 (FORC77_0365) pilF 422238..423962 (+) 1725 WP_131069623.1 GspE/PulE family protein Machinery gene
  FORC77_RS02025 (FORC77_0366) - 423972..425192 (+) 1221 WP_011079398.1 type II secretion system F family protein -
  FORC77_RS02030 (FORC77_0367) - 425196..425657 (+) 462 WP_011151248.1 hypothetical protein -
  FORC77_RS02035 (FORC77_0368) - 425736..426314 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC77_RS02040 (FORC77_0369) - 426348..426818 (+) 471 WP_045622012.1 type II secretion system protein -
  FORC77_RS02045 (FORC77_0370) - 426874..427359 (+) 486 WP_045596378.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC77_RS02050 (FORC77_0371) - 427359..427925 (+) 567 WP_045622015.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC77_RS02055 (FORC77_0372) - 427925..428614 (+) 690 WP_131069624.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63702.06 Da        Isoelectric Point: 5.3226

>NTDB_id=229936 FORC77_RS02020 WP_131069623.1 422238..423962(+) (pilF) [Vibrio vulnificus strain FORC_077]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVSASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=229936 FORC77_RS02020 WP_131069623.1 422238..423962(+) (pilF) [Vibrio vulnificus strain FORC_077]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCGCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GACAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGCGATGAAGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGAGCGCATCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGTCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCAGAAAAACGTCTGCCACAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCAGGTTTGCCGGACGATCTTTTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACGGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAGTTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAATCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATTATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTGCACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGACATGGGTGCTCCGGGCTATCTGGTTGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACGGAAGATCATGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGTGGTTGTCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTTGAGTTTGCACATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.522

98.432

0.369