Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ALW85_RS00360 Genome accession   NC_004757
Coordinates   81662..83125 (-) Length   487 a.a.
NCBI ID   WP_011110714.1    Uniprot ID   Q82Y27
Organism   Nitrosomonas europaea ATCC 19718     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76662..88125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALW85_RS00330 (NE0056) mutY 77027..78160 (+) 1134 WP_011110708.1 A/G-specific adenine glycosylase -
  ALW85_RS00335 (NE0057) - 78153..78779 (+) 627 WP_011110709.1 5-formyltetrahydrofolate cyclo-ligase -
  ALW85_RS00340 (NE0058) - 78837..79451 (-) 615 WP_041356264.1 flavin prenyltransferase UbiX -
  ALW85_RS00345 (NE0059) hprK 79491..80462 (-) 972 WP_011110711.1 HPr(Ser) kinase/phosphatase -
  ALW85_RS00350 (NE0060) - 80449..80919 (-) 471 WP_011110712.1 PTS sugar transporter subunit IIA -
  ALW85_RS00355 (NE0061) raiA 81151..81486 (-) 336 WP_011110713.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ALW85_RS00360 (NE0062) rpoN/rpoN1 81662..83125 (-) 1464 WP_011110714.1 RNA polymerase factor sigma-54 Machinery gene
  ALW85_RS00365 (NE0063) lptB 83132..83854 (-) 723 WP_011110715.1 LPS export ABC transporter ATP-binding protein -
  ALW85_RS00370 (NE0064) lptA 83889..84383 (-) 495 WP_234988943.1 lipopolysaccharide transport periplasmic protein LptA -
  ALW85_RS00375 (NE0065) lptC 84563..85144 (-) 582 WP_011110717.1 LPS export ABC transporter periplasmic protein LptC -
  ALW85_RS00380 (NE0066) nadA 85429..86529 (+) 1101 WP_011110718.1 quinolinate synthase NadA -
  ALW85_RS00385 (NE0067) - 86529..87353 (+) 825 WP_011110719.1 endonuclease/exonuclease/phosphatase family protein -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54948.38 Da        Isoelectric Point: 5.0786

>NTDB_id=22968 ALW85_RS00360 WP_011110714.1 81662..83125(-) (rpoN/rpoN1) [Nitrosomonas europaea ATCC 19718]
MKPTLQLKLNTGLTLTPQLQQSIRLLQLSTLELNQEITRLVQENPLLELDEGIDQDGFETGGPDISLSPSDSSAATESNG
ISEIGKNTDIQSDWPDDPLYADQEFFFDTRRRSDEDHDQDFSRLISKPVSLREHLLSQISLGQYCERSKKIAELLVDSLN
DDGYLSQDLDELAELLPPELEISTADLESALDYIQQLDPPGVGARNLQECLSLQLKALPGNTPLRDEALKLVNGHLESFA
AKNFQLLKKVLDCNETCLQSIYQLITQLNPKPGDDFNTTTARHVIPDVIVTRSDSGWGVRLNSDSVPRVRINSLYAGILK
HHRTEATQMLMGQLKEARWLVRNLDQRMDTILRVSQAIVDCQQAFLEQGETAIRPLVMREIAVTLGLHESTISRVTTQKY
MRTPHGIFELKYFFGSHIPAERGEAHSAIAIRGLIKRLIQDEDRRKPLNDSQISQMLAQQGIVIARRTVAKYREFMHIPP
TNLRKTL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=22968 ALW85_RS00360 WP_011110714.1 81662..83125(-) (rpoN/rpoN1) [Nitrosomonas europaea ATCC 19718]
ATGAAACCAACCCTGCAACTCAAGCTCAACACCGGCCTTACGCTGACACCGCAGTTACAGCAATCTATCCGGTTACTGCA
ACTGTCTACGCTCGAGCTCAATCAGGAAATCACGCGTCTCGTACAGGAAAACCCCCTGCTCGAACTGGATGAAGGGATTG
ATCAGGATGGATTTGAGACTGGAGGACCGGATATTTCCCTGTCACCTTCAGATTCGTCTGCTGCAACAGAAAGCAATGGA
ATCTCAGAAATCGGAAAAAATACAGATATCCAGAGTGATTGGCCGGATGACCCGTTATATGCCGATCAGGAATTTTTCTT
CGATACACGTAGGCGGAGTGATGAAGACCATGACCAGGATTTCTCGCGCTTGATCAGCAAACCCGTCAGTCTGCGGGAAC
ACTTGCTCTCACAAATCAGTCTAGGGCAATACTGTGAGCGCAGTAAGAAAATCGCCGAACTGCTGGTGGACAGCCTCAAT
GACGATGGGTATTTGTCTCAAGATCTGGATGAGCTTGCGGAATTGCTACCACCCGAATTGGAAATCAGTACCGCAGATCT
GGAGTCAGCTCTGGATTATATTCAGCAACTGGATCCACCGGGTGTGGGGGCGCGCAATCTGCAGGAATGTCTGTCCTTAC
AACTTAAAGCATTACCGGGCAACACGCCATTGCGAGATGAAGCGCTCAAACTGGTAAACGGGCATCTGGAGAGTTTTGCT
GCTAAAAATTTCCAATTACTCAAAAAGGTGCTTGATTGTAATGAAACTTGTCTGCAATCGATCTACCAGCTGATTACTCA
ACTCAATCCCAAGCCTGGTGATGATTTCAATACGACAACAGCACGTCATGTCATCCCGGACGTGATCGTGACCCGGTCAG
ACAGCGGCTGGGGGGTACGGCTCAACTCCGATTCGGTTCCGCGCGTGCGCATCAACAGCTTGTATGCCGGTATTTTGAAA
CACCATCGCACGGAAGCAACTCAGATGCTGATGGGTCAGTTGAAAGAGGCCCGCTGGCTGGTCAGAAATCTCGATCAACG
GATGGATACCATACTCCGGGTCAGTCAGGCGATTGTCGATTGTCAGCAAGCTTTCCTCGAACAGGGCGAAACTGCTATAC
GTCCTTTAGTTATGCGCGAAATTGCTGTGACACTTGGGCTGCACGAATCGACCATTTCGCGCGTTACGACTCAGAAATAC
ATGCGGACACCGCACGGTATTTTTGAGCTCAAATATTTTTTTGGCAGCCATATCCCGGCTGAAAGGGGAGAAGCACATTC
AGCCATTGCCATCCGTGGGCTGATCAAGCGGCTGATTCAAGATGAAGATCGCAGAAAACCGCTCAATGACAGTCAGATTT
CACAGATGCTGGCGCAGCAGGGTATTGTTATTGCTCGCCGTACGGTTGCAAAATATCGGGAGTTCATGCATATTCCCCCG
ACTAATTTACGCAAAACACTCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(122-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q82Y27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.863

100

0.491

Multiple sequence alignment