Detailed information NTG0229327

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: CA926_RS03490
• Genome accession: NZ_CP021265
• Coordinates: 722739..723188 (-)
• Length: 149 a.a.
• NCBI ID: WP_027228447.1
• Uniprot ID: A0A2S6F4D1
• Organism: Legionella pneumophila subsp. fraseri strain Los Angeles
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 717739..728188

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CA926_RS03470	-	718011..718403 (-)	393	WP_027228443.1	hypothetical protein	-
CA926_RS03475	-	718540..718917 (+)	378	WP_027228444.1	hypothetical protein	-
CA926_RS03480	-	719117..720406 (+)	1290	WP_027228445.1	tetratricopeptide repeat protein	-
CA926_RS03485	comEC	720454..722661 (-)	2208	WP_028379724.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CA926_RS03490	pilE	722739..723188 (-)	450	WP_027228447.1	type IV pilin protein	Machinery gene
CA926_RS03495	-	723200..726709 (-)	3510	WP_028379725.1	PilC/PilY family type IV pilus protein	-
CA926_RS03500	-	726722..727234 (-)	513	WP_027228449.1	pilus assembly protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16426.72 Da
• Isoelectric Point: 8.4634

>NTDB_id=229327 CA926_RS03490 WP_027228447.1 722739..723188(-) (pilE) [Legionella pneumophila subsp. fraseri strain Los Angeles]
MLNNRHFMKNKRMRQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=229327 CA926_RS03490 WP_027228447.1 722739..723188(-) (pilE) [Legionella pneumophila subsp. fraseri strain Los Angeles]
ATGCTCAATAATCGACATTTTATGAAGAATAAGCGCATGAGACAATCTGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAGTCCCGTCGTGCCGATGCCC
ATGCTACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCTGCGGCGTGTGGCGCC
TTACCGGCATTTCCGCAAACAACACCAAATGGGTACTATACGATCAATATTTCAAACCTTACAGCTACAACGTATACCTT
AACGGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATCAATCAAGCGAATGTAAAAACAG
CAGTAGATTCCTCTGCTAATGCCCAGCCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: A0A2S6F4D1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	96.644	100	0.966
pilE	Legionella pneumophila strain ERS1305867	96.644	100	0.966