Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BC_RS19975 Genome accession   NC_004722
Coordinates   3980145..3980579 (-) Length   144 a.a.
NCBI ID   WP_000811496.1    Uniprot ID   Q819H5
Organism   Bacillus cereus ATCC 14579     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3975145..3985579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC_RS19960 (BC4001) - 3976321..3976878 (+) 558 WP_000062059.1 PadR family transcriptional regulator -
  BC_RS19965 (BC4003) metE 3977392..3979680 (+) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BC_RS19970 (BC4004) comJ 3979719..3980108 (-) 390 WP_000424030.1 competence protein ComJ -
  BC_RS19975 (BC4005) nucA/comI 3980145..3980579 (-) 435 WP_000811496.1 DNA-entry nuclease Machinery gene
  BC_RS19980 (BC4006) - 3980806..3981555 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  BC_RS19985 (BC4007) kinB 3981594..3982868 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  BC_RS19990 (BC4008) - 3983179..3983379 (+) 201 WP_000929278.1 hypothetical protein -
  BC_RS19995 (BC4009) - 3983502..3984665 (+) 1164 WP_000434582.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16085.45 Da        Isoelectric Point: 8.4331

>NTDB_id=22931 BC_RS19975 WP_000811496.1 3980145..3980579(-) (nucA/comI) [Bacillus cereus ATCC 14579]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAQYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=22931 BC_RS19975 WP_000811496.1 3980145..3980579(-) (nucA/comI) [Bacillus cereus ATCC 14579]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACAATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q819H5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment