Detailed information NTG0228942

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CAC37_RS07195
• Genome accession: NZ_CP021178
• Coordinates: 1525569..1526066 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus subsp. aureus ST398 strain 2012-3
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1520569..1531066

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CAC37_RS07170 (CAC37_07470)	gcvPB	1520731..1522203 (-)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
CAC37_RS07175 (CAC37_07475)	gcvPA	1522196..1523542 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
CAC37_RS07180 (CAC37_07480)	gcvT	1523562..1524653 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
CAC37_RS07185 (CAC37_07485)	-	1524812..1525336 (-)	525	WP_001015120.1	shikimate kinase	-
CAC37_RS07190 (CAC37_07490)	-	1525326..1525472 (-)	147	WP_001789879.1	hypothetical protein	-
CAC37_RS07195 (CAC37_07495)	comGF	1525569..1526066 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
CAC37_RS07200 (CAC37_07500)	comGE	1525984..1526283 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
CAC37_RS07205 (CAC37_07505)	comGD	1526270..1526716 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
CAC37_RS07210 (CAC37_07510)	comGC	1526694..1527005 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
CAC37_RS07215 (CAC37_07515)	comGB	1527019..1528089 (-)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
CAC37_RS07220 (CAC37_07520)	comGA	1528061..1529035 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
CAC37_RS07225 (CAC37_07525)	-	1529087..1529710 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
CAC37_RS07230 (CAC37_07530)	-	1529707..1530036 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
CAC37_RS07235 (CAC37_07535)	-	1530036..1531022 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=228942 CAC37_RS07195 WP_001825340.1 1525569..1526066(-) (comGF) [Staphylococcus aureus subsp. aureus ST398 strain 2012-3]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=228942 CAC37_RS07195 WP_001825340.1 1525569..1526066(-) (comGF) [Staphylococcus aureus subsp. aureus ST398 strain 2012-3]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97