Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   AM456_RS11535 Genome accession   NZ_CP026755
Coordinates   2029515..2030942 (+) Length   475 a.a.
NCBI ID   WP_000980568.1    Uniprot ID   B7L9N7
Organism   Escherichia coli strain AR_0077     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2024515..2035942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM456_RS11505 (AM456_11520) pduU 2024892..2025242 (+) 351 WP_000211397.1 propanediol utilization microcompartment protein PduU -
  AM456_RS11510 (AM456_11525) - 2025247..2025690 (+) 444 WP_094029870.1 EutP/PduV family microcompartment system protein -
  AM456_RS11515 (AM456_11530) tmaR 2025791..2026120 (-) 330 WP_000450409.1 DUF496 family protein -
  AM456_RS11520 (AM456_11535) yeeA 2026292..2027350 (-) 1059 WP_001200890.1 FUSC family protein -
  AM456_RS11525 (AM456_11540) sbmC 2027548..2028021 (-) 474 WP_094029869.1 DNA gyrase inhibitor SbmC -
  AM456_RS11530 (AM456_11545) dacD 2028140..2029306 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  AM456_RS11535 (AM456_11555) sbcB 2029515..2030942 (+) 1428 WP_000980568.1 exodeoxyribonuclease I Machinery gene
  AM456_RS11540 (AM456_11560) plaP 2031016..2032374 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  AM456_RS11545 (AM456_11565) yoeI 2032364..2032426 (-) 63 WP_010723108.1 membrane protein YoeI -
  AM456_RS11550 (AM456_11570) yeeY 2032641..2033570 (-) 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
  AM456_RS11555 (AM456_11575) yeeZ 2033616..2034440 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  AM456_RS11560 (AM456_11580) yoeB 2034523..2034777 (-) 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  AM456_RS11565 (AM456_11585) yefM 2034774..2035025 (-) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  AM456_RS11570 (AM456_11590) hisL 2035308..2035358 (+) 51 WP_001364200.1 his operon leader peptide -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54485.76 Da        Isoelectric Point: 5.4700

>NTDB_id=228545 AM456_RS11535 WP_000980568.1 2029515..2030942(+) (sbcB) [Escherichia coli strain AR_0077]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EATFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=228545 AM456_RS11535 WP_000980568.1 2029515..2030942(+) (sbcB) [Escherichia coli strain AR_0077]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCACGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAGCATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTTGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L9N7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583