Detailed information NTG0228443

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: MTAI_RS03570
• Genome accession: NZ_CP021130
• Coordinates: 699999..702656 (+)
• Length: 885 a.a.
• NCBI ID: WP_043983074.1
• Uniprot ID: -
• Organism: Meiothermus taiwanensis WR-220
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 694999..707656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTAI_RS03550 (Mtai_v1c06960)	gap	696072..697064 (-)	993	WP_043983065.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
MTAI_RS03555 (Mtai_v1c06970)	-	697233..698393 (-)	1161	WP_187388591.1	A24 family peptidase	-
MTAI_RS03560 (Mtai_v1c06980)	pgeF	698570..699304 (+)	735	WP_013013231.1	peptidoglycan editing factor PgeF	-
MTAI_RS03565 (Mtai_v1c06990)	-	699342..699899 (+)	558	WP_187388592.1	YqeG family HAD IIIA-type phosphatase	-
MTAI_RS03570 (Mtai_v1c07010)	pilF	699999..702656 (+)	2658	WP_043983074.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
MTAI_RS03575 (Mtai_v1c07020)	-	702765..704123 (+)	1359	WP_043983076.1	dipeptidase	-
MTAI_RS03580 (Mtai_v1c07030)	-	704442..705671 (+)	1230	WP_043983078.1	PEGA domain-containing protein	-
MTAI_RS03585 (Mtai_v1c07040)	-	705891..706799 (-)	909	WP_027887222.1	manganese catalase family protein	-

Sequence

Protein

• Length: 885 a.a.
• Molecular weight: 97965.73 Da
• Isoelectric Point: 5.0589

>NTDB_id=228443 MTAI_RS03570 WP_043983074.1 699999..702656(+) (pilF) [Meiothermus taiwanensis WR-220]
MANVLTIGDKRLGAALLDMGLLGDEELQRALEQHREVGGNLSEIIVELGLLSERRIAQAIEQAFGIPLVELAEMEISPEA
KSLIPAERARDLGAIPFSVEGGTLRVALLNPLDNLVLEELEDLTNQIIEPYLATQSSFRYALAVHYPELGLPVPPPPSAA
SPGEMQLGEILVSKGWLSREDLEAALVEQEKTGELLGRLLVNKFGLAEERLYQALAEQAGIEFRAELDDLELQPEVSAFL
LRPDALRYHAIPARQDGQQVLVVLADPRHRHTVARLIEHPTKFILTLPKVWETLFSKAYPEKSRLGEALVQEGKLGRAAL
QDALSVQRRMGKTRPLGEVLVELGYVSAQDVEEALAKQRQGGGRLEDTLVQSGKIKPEMLAKSLATQLGYPYIDPTDFPP
DPSVLTMVPESTVRRYTIFPHHLEGNTLVVLMKDPRNILAIDDLRMITKRDVMPAVSAEAAITKLIERFYGSTTGVEELA
REFEGKKKEEESTDTSALDDNAVVKLVNSIIREAFLQDASDIHIEPRQSDILVRIRIDGTLREYMRLPKGAGPAIASRIK
IMANLDIAERRLPQDGRVRYRDRSIDLDLRLSTLPTVYGEKIVMRLLRKAADIPEIEQLGFAPDVFQRFEDVISKPYGIF
LITGPTGSGKSFTTFSILKRIATPDKNTTTIEDPVEYEIPGINQTQVNPVAGLTFAKALRSFLRQDPDIIMVGEIRDSET
AKIATEAALTGHLVIATLHTNDAAGAVTRLDEMGVELFNISAALVGVLAQRLVRKICEHCKIQIEPDPTVLRRLDLSKED
LRGKTLYKGTGCDKCNGTGYKGRTAIHELMVLDDEIRRAIVEGQSATQIKEIARRGGMKTLREDGIQKAFLGLTTLEEVM
ARTNE

Nucleotide

• Length: 2658 bp

>NTDB_id=228443 MTAI_RS03570 WP_043983074.1 699999..702656(+) (pilF) [Meiothermus taiwanensis WR-220]
ATGGCTAACGTCTTAACGATTGGAGACAAACGGCTGGGGGCCGCCCTGTTGGACATGGGGCTGCTGGGCGATGAGGAACT
GCAGCGAGCCCTCGAGCAGCACCGTGAGGTGGGGGGTAACCTCTCAGAGATTATCGTCGAATTGGGCTTGCTTTCTGAGC
GGCGCATCGCCCAGGCCATTGAGCAAGCTTTTGGTATCCCGCTGGTGGAGTTGGCCGAAATGGAAATTTCACCCGAGGCC
AAGTCGCTCATTCCAGCAGAGCGGGCGCGTGACCTGGGTGCCATTCCCTTTAGCGTGGAGGGGGGTACCCTGCGGGTGGC
CCTGCTCAACCCGCTGGACAACCTGGTGCTCGAGGAGCTTGAAGATCTCACCAATCAAATCATTGAGCCCTACCTGGCCA
CCCAGTCCTCTTTTCGCTACGCCCTGGCAGTGCACTACCCCGAGCTGGGCCTGCCGGTGCCCCCGCCGCCCTCGGCTGCC
TCGCCGGGCGAGATGCAACTGGGTGAAATCCTGGTCAGCAAAGGCTGGCTGAGCCGGGAGGATCTCGAGGCTGCTTTAGT
CGAGCAGGAGAAGACCGGTGAGCTACTAGGTCGGCTGCTTGTCAACAAATTCGGCCTGGCTGAGGAGCGGCTCTACCAGG
CCCTGGCCGAACAGGCCGGCATCGAGTTTAGAGCAGAACTGGACGACCTCGAGCTGCAACCCGAGGTCTCGGCCTTTTTG
TTGCGCCCCGATGCGCTGCGCTACCATGCCATACCAGCCCGTCAGGATGGGCAGCAGGTGCTGGTGGTGCTGGCCGATCC
GCGCCACCGACACACCGTGGCCCGGTTGATTGAACACCCTACCAAGTTCATCCTCACCCTGCCCAAGGTTTGGGAAACCC
TGTTTAGCAAGGCCTACCCTGAAAAAAGCCGCCTGGGGGAAGCGCTGGTGCAGGAAGGCAAGCTGGGGCGCGCTGCTTTG
CAGGATGCGCTTTCGGTGCAGCGCCGCATGGGAAAAACCCGGCCTCTGGGTGAGGTTTTGGTGGAGCTGGGCTACGTGAG
TGCCCAGGATGTGGAAGAAGCCCTGGCGAAACAGCGGCAGGGTGGGGGTCGCCTCGAGGATACCCTGGTGCAGTCGGGCA
AAATCAAGCCGGAGATGCTGGCCAAGAGCCTGGCTACCCAGCTTGGCTACCCCTACATCGACCCCACCGACTTCCCCCCC
GACCCTTCGGTGCTCACCATGGTGCCCGAGTCCACCGTGCGGCGCTACACCATCTTCCCGCACCACCTCGAGGGCAACAC
CCTGGTGGTCTTGATGAAAGACCCCCGCAACATCCTGGCTATCGACGACCTGCGCATGATCACCAAGCGCGATGTGATGC
CGGCGGTCTCGGCGGAAGCGGCCATCACCAAGCTCATCGAGCGGTTCTATGGCAGCACCACCGGGGTGGAGGAGCTGGCC
CGCGAGTTCGAGGGCAAGAAGAAAGAGGAGGAGTCCACCGATACCAGCGCCCTCGACGATAACGCGGTGGTCAAGCTGGT
CAACAGCATTATTCGCGAGGCTTTCTTGCAGGATGCTTCGGACATCCACATCGAGCCCCGCCAGTCGGATATCCTGGTGC
GCATCCGAATCGACGGCACCCTGCGGGAGTACATGCGTTTGCCCAAGGGGGCCGGGCCGGCGATTGCCTCGAGGATCAAG
ATTATGGCCAACCTGGACATCGCCGAGCGGCGCTTGCCCCAGGACGGCCGGGTGCGCTACCGCGACCGTTCCATTGACCT
GGATCTGCGCCTTTCCACCCTGCCCACCGTATACGGCGAAAAAATTGTAATGCGCCTGCTGCGCAAGGCCGCCGATATTC
CCGAAATTGAGCAGCTCGGATTTGCGCCGGACGTGTTTCAACGCTTCGAAGACGTGATTTCCAAGCCGTATGGCATCTTC
CTGATTACCGGGCCTACGGGCTCGGGTAAGTCCTTCACCACCTTCAGCATCCTGAAGCGCATCGCCACCCCCGATAAAAA
CACCACCACCATCGAAGACCCGGTGGAGTACGAGATTCCCGGGATTAACCAAACCCAGGTGAACCCGGTGGCGGGCTTGA
CCTTCGCCAAGGCCCTGCGGAGCTTTCTGCGGCAAGACCCCGATATCATTATGGTGGGTGAGATCCGCGACTCTGAAACG
GCCAAAATTGCCACCGAGGCGGCCCTGACCGGCCACCTGGTGATCGCCACCCTGCACACCAACGACGCCGCAGGGGCCGT
TACCCGCCTGGATGAGATGGGGGTGGAGCTTTTCAACATCTCGGCGGCCCTGGTGGGGGTGCTGGCCCAGCGCTTGGTGC
GGAAGATCTGCGAGCACTGCAAAATTCAGATCGAGCCCGACCCAACCGTGCTGCGCCGACTGGATCTGTCTAAAGAGGAT
CTGCGCGGCAAAACCCTCTACAAAGGTACGGGGTGTGATAAGTGCAACGGCACAGGTTATAAGGGCCGCACCGCCATCCA
CGAGCTAATGGTGCTCGACGATGAGATCCGGCGGGCGATTGTGGAGGGGCAGTCGGCCACCCAGATTAAGGAGATCGCCC
GTAGGGGTGGTATGAAAACCCTGCGCGAGGACGGTATTCAAAAAGCCTTCCTGGGGCTGACCACCCTGGAAGAAGTAATG
GCCCGTACCAATGAGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (509-775)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-147)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-473)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (173-303)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	69.326	100	0.697
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.674	99.887	0.566