Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CAH07_RS20930 Genome accession   NZ_CP021123
Coordinates   4016334..4017710 (-) Length   458 a.a.
NCBI ID   WP_029727024.1    Uniprot ID   -
Organism   Bacillus subtilis strain SEM-9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4011334..4022710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAH07_RS20910 (CAH07_20715) ispF 4012850..4013326 (-) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  CAH07_RS20915 (CAH07_20720) ispD 4013319..4014017 (-) 699 WP_046664350.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CAH07_RS20920 (CAH07_20725) yacL 4014032..4015132 (-) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  CAH07_RS20925 (CAH07_20730) disA 4015248..4016330 (-) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  CAH07_RS20930 (CAH07_20735) radA 4016334..4017710 (-) 1377 WP_029727024.1 DNA repair protein RadA Machinery gene
  CAH07_RS20935 (CAH07_20740) clpC 4017802..4020234 (-) 2433 WP_003235011.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  CAH07_RS20940 (CAH07_20745) mcsB 4020231..4021322 (-) 1092 WP_003235007.1 protein arginine kinase -
  CAH07_RS20945 (CAH07_20750) mcsA 4021322..4021879 (-) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  CAH07_RS20950 (CAH07_20755) ctsR 4021893..4022357 (-) 465 WP_003225724.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49452.96 Da        Isoelectric Point: 8.2399

>NTDB_id=228429 CAH07_RS20930 WP_029727024.1 4016334..4017710(-) (radA) [Bacillus subtilis strain SEM-9]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANIAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=228429 CAH07_RS20930 WP_029727024.1 4016334..4017710(-) (radA) [Bacillus subtilis strain SEM-9]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTAACGAAAGAAGGGTCTATTGCAGGTCCAAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTCTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATATTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627

Multiple sequence alignment