Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   CAH07_RS08585 Genome accession   NZ_CP021123
Coordinates   1633334..1634101 (+) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain SEM-9     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1628334..1639101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAH07_RS08555 (CAH07_08440) pgpH 1628630..1630765 (+) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -
  CAH07_RS08560 (CAH07_08445) ybeY 1630766..1631239 (+) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  CAH07_RS08565 (CAH07_08450) unpK 1631220..1631591 (+) 372 WP_015384119.1 diacylglycerol kinase family protein -
  CAH07_RS08570 (CAH07_08455) - 1631710..1632120 (+) 411 WP_003230049.1 cytidine deaminase -
  CAH07_RS08575 (CAH07_08460) era 1632101..1633006 (+) 906 WP_003230051.1 GTPase Era -
  CAH07_RS08580 (CAH07_08465) yqzL 1633154..1633297 (+) 144 WP_003230053.1 YqzL family protein -
  CAH07_RS08585 (CAH07_08470) recO 1633334..1634101 (+) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  CAH07_RS08590 (CAH07_08475) glyQ 1634398..1635285 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  CAH07_RS08595 (CAH07_08480) glyS 1635278..1637317 (+) 2040 WP_029317896.1 glycine--tRNA ligase subunit beta -
  CAH07_RS08600 (CAH07_08485) ccpN 1637450..1638088 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  CAH07_RS08605 (CAH07_08490) ppsR 1638114..1638926 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=228385 CAH07_RS08585 WP_014477369.1 1633334..1634101(+) (recO) [Bacillus subtilis strain SEM-9]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=228385 CAH07_RS08585 WP_014477369.1 1633334..1634101(+) (recO) [Bacillus subtilis strain SEM-9]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAGTCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment