Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CAL13_RS00325 Genome accession   NZ_CP021109
Coordinates   72952..74391 (+) Length   479 a.a.
NCBI ID   WP_086071176.1    Uniprot ID   A0A1W6YV33
Organism   Bordetella genomosp. 9 strain AU17164     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 67952..79391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAL13_RS00305 (CAL13_00305) - 68309..69172 (+) 864 WP_420042429.1 carbohydrate ABC transporter permease -
  CAL13_RS00310 (CAL13_00310) - 69169..70059 (+) 891 WP_086071174.1 carbohydrate ABC transporter permease -
  CAL13_RS00315 (CAL13_00315) leuA 70132..71841 (-) 1710 WP_086055729.1 2-isopropylmalate synthase -
  CAL13_RS00320 (CAL13_00320) - 72222..72953 (-) 732 WP_086055730.1 hypothetical protein -
  CAL13_RS00325 (CAL13_00325) rpoN/rpoN1 72952..74391 (+) 1440 WP_086071176.1 RNA polymerase factor sigma-54 Machinery gene
  CAL13_RS00330 (CAL13_00330) - 74495..74767 (+) 273 WP_086071177.1 (2Fe-2S)-binding protein -
  CAL13_RS00335 (CAL13_00335) - 75133..76050 (+) 918 WP_086071178.1 DMT family transporter -
  CAL13_RS00340 (CAL13_00340) bfr 76248..76724 (+) 477 WP_086055734.1 bacterioferritin -
  CAL13_RS00345 (CAL13_00345) - 76843..77532 (-) 690 WP_086055735.1 hypothetical protein -
  CAL13_RS00350 (CAL13_00350) - 77725..79332 (-) 1608 WP_086071179.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 52624.05 Da        Isoelectric Point: 5.3886

>NTDB_id=228278 CAL13_RS00325 WP_086071176.1 72952..74391(+) (rpoN/rpoN1) [Bordetella genomosp. 9 strain AU17164]
MQNIVPMTRHALELRPGQHLALTPQLQQSIRMLQLSTLDLEAEISQALAENPLLERDDNPAPDDIDANREGSAHEDEPPP
ERETPVDEMPGAKGVYADEDGDMPEAARGETLREHLLAQLALTRAGRRDAALVALLIDELDENGYLGSPLEEILAWLPAE
VEADLDELRAALRLLQSFDPPGIGARDMAECLALQLRHPDAEALPEAADPTVLACARAICAEHLPLLASGNQARLREALL
CDDATARRARALILKLDPRPGRRWTVPAADYAIPDVIVRKSRKGWQVVLNSAAMPRLRVNHIYAQMLGNQREGGHSALHA
QLQQARWMIRNVEQRFDTILRVAQAIVDHQSAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTMELKD
FFGAGVATESGDTASATAVQAFIKRLVAEENRAKPLSDNQIMLKLAEQGIVIARRTVAKYREALRIAPASVRKAQASAR

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=228278 CAL13_RS00325 WP_086071176.1 72952..74391(+) (rpoN/rpoN1) [Bordetella genomosp. 9 strain AU17164]
ATGCAAAATATTGTACCTATGACTCGCCACGCCTTAGAACTTCGGCCAGGACAGCACCTCGCGCTGACCCCGCAGCTGCA
GCAATCCATCCGGATGCTGCAGTTGTCGACGCTCGACCTGGAGGCGGAGATTTCCCAGGCGCTGGCCGAAAACCCCTTGC
TGGAGCGCGACGACAACCCCGCCCCGGACGATATCGACGCCAATCGCGAGGGGTCGGCCCACGAGGACGAGCCGCCGCCG
GAAAGGGAAACCCCGGTGGACGAAATGCCCGGCGCCAAGGGCGTGTACGCGGACGAGGACGGCGACATGCCGGAAGCCGC
GCGGGGCGAAACCCTGCGGGAACACCTGCTGGCGCAATTGGCGCTGACGCGCGCGGGCCGGCGCGACGCCGCTCTGGTGG
CGCTGCTGATCGACGAGCTGGACGAAAACGGCTACCTGGGTTCGCCCCTGGAGGAAATCCTGGCCTGGCTGCCTGCCGAG
GTGGAAGCGGACCTGGACGAGTTGCGCGCCGCCTTGCGCCTGCTGCAGTCCTTCGACCCGCCTGGTATCGGGGCGCGCGA
CATGGCCGAATGCCTGGCGCTGCAGCTGCGGCACCCCGACGCCGAGGCGCTGCCAGAGGCTGCGGATCCGACCGTACTGG
CCTGCGCGCGCGCCATCTGCGCCGAACATCTGCCGTTGCTCGCATCGGGCAATCAGGCCCGGCTGCGGGAGGCGTTGCTT
TGCGACGACGCCACCGCCCGGCGCGCTCGCGCCCTGATCCTGAAGCTGGACCCGCGCCCCGGCCGCCGATGGACCGTGCC
TGCGGCCGACTATGCCATTCCGGACGTCATCGTCCGCAAGAGCCGCAAGGGGTGGCAGGTCGTGTTGAACAGCGCCGCGA
TGCCGCGCCTGCGCGTCAACCACATCTACGCTCAGATGCTCGGCAATCAGCGGGAAGGCGGGCATTCGGCCCTGCACGCG
CAACTGCAGCAGGCGCGCTGGATGATCCGCAATGTCGAGCAGCGCTTCGATACCATCCTGCGGGTTGCCCAGGCCATCGT
CGACCACCAGTCGGCTTTCTTCAGCCAGGGCCCGTCCGCCATGCGGCCCCTCATCCTCAAGGACATCGCAGGTGAGTTGG
GGCTGCACGAGTCTACGATTTCGCGGGCCACCACACAGAAGTACATGCTTACACCGTTCGGCACCATGGAGCTGAAGGAC
TTTTTCGGCGCCGGCGTTGCCACGGAAAGCGGGGACACGGCCTCCGCGACGGCGGTCCAGGCTTTTATCAAACGGCTGGT
GGCGGAAGAAAACCGTGCCAAGCCGCTATCCGACAATCAAATCATGCTCAAGCTGGCGGAGCAGGGCATCGTCATCGCTC
GCCGCACCGTGGCGAAGTATCGTGAGGCGCTGCGTATTGCACCGGCCTCTGTCCGCAAGGCGCAGGCGTCCGCGCGATAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-473)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.209

100

0.52

Multiple sequence alignment