Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EBAPG3_RS02300 Genome accession   NZ_CP021106
Coordinates   509761..511236 (-) Length   491 a.a.
NCBI ID   WP_004175555.1    Uniprot ID   A0A1W6ST50
Organism   Nitrosospira lacus strain APG3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504761..516236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBAPG3_RS02275 (EBAPG3_002265) - 505983..506609 (+) 627 WP_040851523.1 5-formyltetrahydrofolate cyclo-ligase -
  EBAPG3_RS02280 (EBAPG3_002270) - 506688..507302 (-) 615 WP_004175548.1 flavin prenyltransferase UbiX -
  EBAPG3_RS02285 (EBAPG3_002275) rapZ 507409..508248 (-) 840 WP_004175550.1 RNase adapter RapZ -
  EBAPG3_RS02290 (EBAPG3_002280) hprK 508319..509305 (-) 987 WP_004175552.1 HPr(Ser) kinase/phosphatase -
  EBAPG3_RS02295 (EBAPG3_002285) hpf 509408..509740 (-) 333 WP_004175553.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EBAPG3_RS02300 (EBAPG3_002290) rpoN/rpoN1 509761..511236 (-) 1476 WP_004175555.1 RNA polymerase factor sigma-54 Machinery gene
  EBAPG3_RS02305 (EBAPG3_002295) lptB 511288..512010 (-) 723 WP_004175557.1 LPS export ABC transporter ATP-binding protein -
  EBAPG3_RS02310 (EBAPG3_002300) lptA 512032..512580 (-) 549 WP_004175558.1 lipopolysaccharide transport periplasmic protein LptA -
  EBAPG3_RS02315 (EBAPG3_002305) lptC 512659..513237 (-) 579 WP_004175559.1 LPS export ABC transporter periplasmic protein LptC -
  EBAPG3_RS02320 (EBAPG3_002310) kdsC 513234..513758 (-) 525 WP_004175563.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EBAPG3_RS02325 (EBAPG3_002315) ispH 514001..514945 (-) 945 WP_004175567.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  EBAPG3_RS02335 (EBAPG3_002325) - 515309..516184 (-) 876 WP_040851539.1 cobalamin-binding protein -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 55340.94 Da        Isoelectric Point: 5.2649

>NTDB_id=228238 EBAPG3_RS02300 WP_004175555.1 509761..511236(-) (rpoN/rpoN1) [Nitrosospira lacus strain APG3]
MKPTLQFKLSQHLTLTPQLQQSIRLLQLSTVELNQEIERIVQENPLLELDDSMGSDATEYRPTPLDSLAELHAPDLLEAS
GDTSAEMPAVESDSGPKNSFADEADWFSDDGTFRNTRDEDDERDFPQQAAEPPNLREHLNLQLSLSQIGERDKRIVGLLI
DSLNDDGYLAQDLEELVSLLPPELEIDIDALHIALEYLQHLDPPGVGARNLRECLIMQLHALPEDTPFRDRALLLVDRHL
DSLASRDFCAIKKLLHCDDECLRSVQHLITHLNPRPGTAFSSTVARYIIPDVIVTKINDTWVASLNPEAMPRLKINRLYA
DILKRSHDDSARRLASQLHEAKWLIKNVQQRFGTILKVSSAVVDRQRQFFEHGAVAMRPLVLREIAEALELHESTISRVT
TQKFMRTPRGIFELKYFFGSHVATDTGGACSATAIRALIKQMINAENAKKPLSDSQISEILGQQGIVVARRTVAKYRESI
QIPPVNLRKSF

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=228238 EBAPG3_RS02300 WP_004175555.1 509761..511236(-) (rpoN/rpoN1) [Nitrosospira lacus strain APG3]
ATGAAGCCTACCCTCCAGTTTAAGCTGTCGCAGCACCTGACGCTTACTCCCCAATTGCAGCAATCCATACGGTTGCTACA
GCTTTCCACGGTTGAACTGAACCAGGAGATCGAGCGCATCGTGCAGGAAAATCCTCTGCTGGAACTGGATGACAGCATGG
GGAGTGATGCCACCGAATATCGCCCTACCCCTCTCGATAGCCTAGCGGAATTGCACGCTCCGGATTTACTGGAAGCCTCC
GGTGACACCAGCGCAGAGATGCCCGCTGTCGAGAGTGACTCCGGCCCAAAAAACAGCTTTGCCGATGAAGCGGACTGGTT
CAGCGACGACGGTACTTTTCGCAACACGCGGGATGAAGACGATGAGCGCGATTTTCCGCAACAGGCGGCGGAGCCCCCCA
ATCTACGTGAGCATCTTAACTTGCAACTCAGCCTGAGCCAGATCGGTGAGCGCGACAAACGTATTGTGGGTTTACTGATA
GACAGCCTGAATGACGACGGCTATCTTGCCCAGGATCTGGAAGAACTCGTCAGTCTGTTACCGCCGGAGCTTGAAATAGA
TATCGATGCTCTGCACATTGCCCTGGAATATTTGCAGCATCTTGACCCGCCTGGCGTGGGCGCGCGTAATTTGAGGGAAT
GTCTGATCATGCAATTGCACGCCCTGCCGGAAGATACGCCCTTCCGGGACCGCGCGTTACTTTTGGTTGACCGTCATCTC
GATAGTCTGGCGTCACGGGATTTCTGCGCAATCAAAAAGTTATTGCATTGCGACGACGAATGTCTGCGCTCAGTACAGCA
CCTTATCACTCACTTAAATCCGAGACCGGGCACGGCATTCAGTTCGACCGTGGCGCGTTACATTATTCCCGACGTAATCG
TAACGAAAATCAATGATACATGGGTGGCCAGTCTTAACCCGGAGGCAATGCCGCGCCTCAAGATCAACCGTCTCTACGCG
GATATCCTGAAACGGAGCCATGATGATTCCGCGCGTCGGCTTGCGAGTCAATTACATGAAGCCAAATGGCTCATCAAGAA
CGTGCAGCAGCGTTTTGGTACCATACTGAAGGTCTCCTCTGCGGTCGTCGATCGCCAGCGGCAGTTCTTCGAGCACGGCG
CAGTTGCCATGCGTCCATTGGTGTTGCGGGAAATTGCAGAAGCACTTGAATTGCATGAGTCCACAATTTCGCGCGTGACC
ACCCAAAAATTTATGCGTACGCCGCGTGGTATATTCGAGTTAAAGTATTTTTTTGGCAGTCACGTGGCGACCGATACGGG
CGGTGCCTGTTCGGCCACCGCGATTCGCGCGCTGATCAAGCAAATGATAAATGCAGAAAATGCAAAAAAACCATTGAGCG
ATAGCCAGATTTCAGAAATTCTGGGACAGCAAGGCATTGTAGTTGCCCGTCGTACTGTCGCAAAATACCGGGAGTCCATT
CAGATTCCTCCCGTTAATCTCCGTAAATCATTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(127-319)

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-489)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6ST50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.734

100

0.55

Multiple sequence alignment