Detailed information NTG0227809

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: B6257_RS08145
• Genome accession: NZ_CP021011
• Coordinates: 1561766..1562911 (+)
• Length: 381 a.a.
• NCBI ID: WP_101562519.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GFP-2
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1556766..1567911

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B6257_RS08120 (B6257_08075)	-	1557663..1558091 (-)	429	WP_031379330.1	DUF1934 domain-containing protein	-
B6257_RS08125 (B6257_08080)	-	1558245..1558757 (+)	513	Protein_1594	bacteriocin biosynthesis protein AlbG	-
B6257_RS08130 (B6257_08085)	-	1558764..1560140 (-)	1377	WP_071392425.1	YncE family protein	-
B6257_RS08135 (B6257_08090)	-	1560180..1561535 (-)	1356	WP_101562518.1	YncE family protein	-
B6257_RS08145 (B6257_08100)	rapF	1561766..1562911 (+)	1146	WP_101562519.1	Rap family tetratricopeptide repeat protein	Regulator
B6257_RS08150	-	1562895..1563014 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
B6257_RS08155 (B6257_08105)	speB	1563136..1564008 (-)	873	WP_003151054.1	agmatinase	-
B6257_RS08160 (B6257_08110)	speE	1564068..1564898 (-)	831	WP_003151052.1	spermidine synthase	-
B6257_RS08165 (B6257_08115)	-	1565098..1567170 (+)	2073	WP_101562520.1	transglycosylase domain-containing protein	-
B6257_RS08170 (B6257_08120)	-	1567195..1567626 (-)	432	WP_031378563.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45795.29 Da
• Isoelectric Point: 4.9327

>NTDB_id=227809 B6257_RS08145 WP_101562519.1 1561766..1562911(+) (rapF) [Bacillus velezensis strain GFP-2]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDEEKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLENIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDILI
NDCLHFLESKLMYADIEELALDVAKYYYTKEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=227809 B6257_RS08145 WP_101562519.1 1561766..1562911(+) (rapF) [Bacillus velezensis strain GFP-2]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTTGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGAAGAAAAATACAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGAACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGCCATTCACTTCCACAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAGAAAATATAGATAACGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTGGAATATTTGTATATGCCGGATCCAAGAGATATATTAATA
AACGATTGTCTGCATTTTCTTGAAAGTAAATTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACAAAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAAGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (188-254)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.604	100	0.706
rapC	Bacillus subtilis subsp. subtilis str. 168	53.421	99.738	0.533