Detailed information NTG0227599

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: C4J63_RS22460
• Genome accession: NZ_CP026612
• Coordinates: 4367027..4368019 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081586.1
• Uniprot ID: Q540P1
• Organism: Escherichia coli strain DTU-1
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 4362027..4373019

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4J63_RS22440 (C4J63_22440)	otnK	4362803..4363969 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
C4J63_RS22445 (C4J63_22445)	ygbL	4364061..4364699 (+)	639	WP_001278994.1	aldolase	-
C4J63_RS22450 (C4J63_22450)	ygbM	4364704..4365480 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
C4J63_RS22455 (C4J63_22455)	ygbN	4365569..4366933 (+)	1365	WP_000104441.1	GntP family transporter	-
C4J63_RS22460 (C4J63_22460)	rpoS	4367027..4368019 (-)	993	WP_000081586.1	RNA polymerase sigma factor RpoS	Regulator
C4J63_RS22465 (C4J63_22465)	nlpD	4368082..4369176 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
C4J63_RS22470 (C4J63_22470)	pcm	4369361..4369987 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
C4J63_RS22475 (C4J63_22475)	surE	4369981..4370742 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
C4J63_RS22480 (C4J63_22480)	truD	4370723..4371772 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
C4J63_RS22485 (C4J63_22485)	ispF	4371769..4372248 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
C4J63_RS22490 (C4J63_22490)	ispD	4372248..4372958 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37956.89 Da
• Isoelectric Point: 4.5886

>NTDB_id=227599 C4J63_RS22460 WP_000081586.1 4367027..4368019(-) (rpoS) [Escherichia coli strain DTU-1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVELEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=227599 C4J63_RS22460 WP_000081586.1 4367027..4368019(-) (rpoS) [Escherichia coli strain DTU-1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAATTGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: Q540P1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715