Detailed information NTG0227130

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: C3F40_RS21315
• Genome accession: NZ_CP026399
• Coordinates: 4006266..4006925 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain ECONIH4
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 4001266..4011925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C3F40_RS21290 (C3F40_21290)	ygiN	4002167..4002481 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
C3F40_RS21295 (C3F40_21295)	mdaB	4002512..4003093 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
C3F40_RS31995	-	4003343..4003822 (+)	480	WP_000065333.1	Hcp family type VI secretion system effector	-
C3F40_RS32000	-	4003825..4004535 (+)	711	WP_000834029.1	hypothetical protein	-
C3F40_RS21305 (C3F40_21305)	ygiZ	4004542..4004874 (+)	333	WP_000914691.1	DUF2645 family protein	-
C3F40_RS21310 (C3F40_21310)	qseC	4004920..4006269 (-)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
C3F40_RS21315 (C3F40_21315)	ciaR	4006266..4006925 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
C3F40_RS21320 (C3F40_21320)	ygiW	4007077..4007469 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
C3F40_RS21325 (C3F40_21325)	ygiV	4007522..4008004 (+)	483	WP_000609258.1	GyrI-like domain-containing protein	-
C3F40_RS21330 (C3F40_21330)	mqsR	4008209..4008505 (+)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
C3F40_RS21335 (C3F40_21335)	mqsA	4008507..4008902 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
C3F40_RS21345 (C3F40_21345)	ygiS	4009035..4010642 (+)	1608	WP_104457550.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=227130 C3F40_RS21315 WP_001221493.1 4006266..4006925(-) (ciaR) [Escherichia coli strain ECONIH4]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=227130 C3F40_RS21315 WP_001221493.1 4006266..4006925(-) (ciaR) [Escherichia coli strain ECONIH4]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365