Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B9L64_RS15210 Genome accession   NZ_CP020915
Coordinates   2946478..2946927 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain 50-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2941478..2951927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9L64_RS15180 yckA 2941629..2942309 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  B9L64_RS15185 yckB 2942319..2943182 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  B9L64_RS21095 - 2943343..2943540 (+) 198 WP_128992271.1 hypothetical protein -
  B9L64_RS15190 yckC 2943560..2944015 (+) 456 WP_048654748.1 RDD family protein -
  B9L64_RS15195 yckD 2944097..2944429 (+) 333 WP_015252849.1 YckD family protein -
  B9L64_RS15200 bglC 2944583..2946016 (+) 1434 WP_048654749.1 6-phospho-beta-glucosidase -
  B9L64_RS15205 nin/comJ 2946053..2946451 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  B9L64_RS15210 nucA/comI 2946478..2946927 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  B9L64_RS15215 tlpC 2947095..2948816 (-) 1722 WP_061891260.1 methyl-accepting chemotaxis protein TlpC -
  B9L64_RS15220 hxlB 2948927..2949484 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  B9L64_RS15225 hxlA 2949490..2950122 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  B9L64_RS15230 hxlR 2950355..2950717 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=227103 B9L64_RS15210 WP_003234608.1 2946478..2946927(-) (nucA/comI) [Bacillus subtilis strain 50-1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=227103 B9L64_RS15210 WP_003234608.1 2946478..2946927(-) (nucA/comI) [Bacillus subtilis strain 50-1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATAATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment