Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   B9L64_RS04930 Genome accession   NZ_CP020915
Coordinates   993856..994623 (-) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis strain 50-1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 988856..999623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9L64_RS04910 ppsR 989031..989843 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  B9L64_RS04915 ccpN 989869..990507 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  B9L64_RS04920 glyS 990640..992679 (-) 2040 WP_003230059.1 glycine--tRNA ligase subunit beta -
  B9L64_RS04925 glyQ 992672..993559 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  B9L64_RS04930 recO 993856..994623 (-) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  B9L64_RS04935 yqzL 994660..994803 (-) 144 WP_003230053.1 YqzL family protein -
  B9L64_RS04940 era 994951..995856 (-) 906 WP_003230051.1 GTPase Era -
  B9L64_RS04945 - 995837..996247 (-) 411 WP_032722130.1 cytidine deaminase -
  B9L64_RS04950 unpK 996366..996737 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  B9L64_RS04955 ybeY 996718..997191 (-) 474 WP_032722131.1 rRNA maturation RNase YbeY -
  B9L64_RS04960 pgpH 997192..999327 (-) 2136 WP_110109423.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=227066 B9L64_RS04930 WP_003230055.1 993856..994623(-) (recO) [Bacillus subtilis strain 50-1]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=227066 B9L64_RS04930 WP_003230055.1 993856..994623(-) (recO) [Bacillus subtilis strain 50-1]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment