Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B9C53_RS01530 Genome accession   NZ_CP020874
Coordinates   336165..336599 (-) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain GYL4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 331165..341599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9C53_RS01495 (B9C53_01465) - 331181..331534 (+) 354 WP_007410379.1 DMT family transporter -
  B9C53_RS01500 (B9C53_01470) - 331548..332000 (-) 453 WP_038463918.1 OsmC family protein -
  B9C53_RS01505 (B9C53_01475) - 332060..332767 (-) 708 WP_038463915.1 poly-gamma-glutamate hydrolase family protein -
  B9C53_RS19740 (B9C53_01480) - 333023..333254 (-) 232 Protein_300 hypothetical protein -
  B9C53_RS01515 (B9C53_01485) - 333433..334761 (+) 1329 WP_038463909.1 S8 family peptidase -
  B9C53_RS01520 (B9C53_01490) - 334954..335316 (+) 363 WP_007410383.1 hypothetical protein -
  B9C53_RS01525 (B9C53_01495) - 335350..336105 (-) 756 WP_003154084.1 YoaK family protein -
  B9C53_RS01530 (B9C53_01500) nucA/comI 336165..336599 (-) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  B9C53_RS01535 (B9C53_01505) - 336888..338099 (+) 1212 WP_038463906.1 cytochrome P450 family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=226738 B9C53_RS01530 WP_007611576.1 336165..336599(-) (nucA/comI) [Bacillus velezensis strain GYL4]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=226738 B9C53_RS01530 WP_007611576.1 336165..336599(-) (nucA/comI) [Bacillus velezensis strain GYL4]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATAGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment