Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B9D94_RS02145 Genome accession   NZ_CP020866
Coordinates   161787..163154 (+) Length   455 a.a.
NCBI ID   WP_006212955.1    Uniprot ID   A0A163HTJ8
Organism   Paenibacillus sp. Cedars     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 156787..168154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9D94_RS02130 (B9D94_02170) - 157096..157620 (+) 525 WP_006212958.1 UvrB/UvrC motif-containing protein -
  B9D94_RS02135 (B9D94_02175) - 157659..158723 (+) 1065 WP_006212957.1 protein arginine kinase -
  B9D94_RS02140 (B9D94_02180) clpC 158833..161289 (+) 2457 WP_006212956.1 ATP-dependent protease ATP-binding subunit ClpC -
  B9D94_RS31725 - 161396..161521 (+) 126 WP_268741817.1 hypothetical protein -
  B9D94_RS02145 (B9D94_02185) radA 161787..163154 (+) 1368 WP_006212955.1 DNA repair protein RadA Machinery gene
  B9D94_RS02150 (B9D94_02190) disA 163168..164244 (+) 1077 WP_006212954.1 DNA integrity scanning diadenylate cyclase DisA -
  B9D94_RS02155 (B9D94_02195) pssA 164295..165032 (+) 738 WP_006212953.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  B9D94_RS02160 (B9D94_02200) - 165165..165560 (-) 396 WP_036638413.1 hypothetical protein -
  B9D94_RS02165 (B9D94_02205) - 165903..166991 (+) 1089 WP_006212951.1 PIN/TRAM domain-containing protein -
  B9D94_RS02170 (B9D94_02210) ispD 167066..167764 (+) 699 WP_172963895.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49538.30 Da        Isoelectric Point: 7.4126

>NTDB_id=226643 B9D94_RS02145 WP_006212955.1 161787..163154(+) (radA) [Paenibacillus sp. Cedars]
MAKTKTKFFCTECGYEAPKWFGKCPGCQSWNSMVEETETVIKTQGMNSPLFHSKEKPLSIINIESGKEPRIQTGIAELNR
VLGGGLVPGSLVLVGGDPGIGKSTLLLQASNRLAQNGVRVLYISGEESVRQTKLRADRLGALSPELFVLCETNLESIEEA
IESVQPGFVVIDSIQTVYLPEVTSAPGSVSQVRECTARFMRLAKVKGIATVLVGHVTKEGAIAGPRMLEHMVDCVLYFEG
ERHHTYRLLRAVKNRFGSTNEIGIFEMGEDGLREVGNPSELFLSERPLGVAGSTVVASMEGTRPVLVELQALISTTHFPS
PRRMGTGVDHHRMNLIIAVLEKRMGMFLQTQDAYLNVAGGVKLDEPAVDLAIAVSIASSFRDIPTKPYDVIFGEVGLTGE
VRAVSRAEQRVREAEKLGFKRVLMPEKSLKGWKHPKGIQIIGVNTVADALAVALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=226643 B9D94_RS02145 WP_006212955.1 161787..163154(+) (radA) [Paenibacillus sp. Cedars]
ATGGCAAAAACTAAGACGAAATTTTTCTGTACCGAATGCGGCTATGAAGCGCCCAAATGGTTCGGAAAATGTCCGGGCTG
CCAATCATGGAATTCCATGGTAGAAGAGACCGAGACGGTAATCAAGACGCAGGGGATGAATTCCCCTCTTTTTCATAGTA
AAGAAAAACCCCTTTCGATCATAAATATAGAAAGCGGCAAAGAACCCAGAATCCAGACGGGCATTGCGGAATTGAACCGT
GTGCTGGGCGGCGGGCTTGTCCCGGGCTCGCTCGTGTTGGTTGGCGGCGATCCCGGCATCGGCAAATCCACGCTGCTTCT
GCAAGCCTCCAACCGATTGGCTCAGAACGGCGTCCGCGTTCTGTATATTTCCGGTGAAGAATCGGTACGTCAGACGAAGC
TTCGCGCTGACCGGCTCGGCGCATTGTCTCCAGAGCTGTTTGTCCTCTGCGAAACCAATCTGGAATCGATTGAGGAGGCC
ATTGAGAGCGTACAGCCCGGTTTTGTTGTCATCGATTCCATTCAAACCGTGTATTTGCCGGAGGTAACAAGCGCGCCGGG
CAGCGTCTCCCAAGTTCGGGAGTGCACCGCCCGCTTTATGCGGCTGGCTAAGGTGAAGGGCATCGCTACGGTTCTTGTTG
GTCATGTGACCAAGGAAGGCGCCATTGCAGGTCCTAGGATGCTGGAGCACATGGTGGACTGTGTGCTTTATTTTGAAGGA
GAGCGTCACCATACTTACCGTTTGCTTCGTGCGGTGAAGAATCGTTTCGGTTCGACGAATGAAATTGGCATATTTGAGAT
GGGTGAGGATGGGCTGCGCGAAGTCGGCAATCCATCGGAACTATTTTTATCTGAACGACCATTAGGCGTAGCCGGTTCGA
CAGTTGTTGCAAGCATGGAGGGAACCCGGCCGGTTCTTGTCGAACTGCAGGCACTCATTTCTACGACCCATTTTCCATCA
CCACGTCGAATGGGCACCGGTGTGGATCATCACCGAATGAATTTAATCATTGCTGTGCTTGAAAAAAGGATGGGCATGTT
CCTGCAAACACAGGATGCCTACCTGAATGTCGCCGGTGGCGTTAAGCTCGATGAACCTGCGGTGGACTTGGCAATAGCGG
TCAGCATTGCATCCAGCTTCCGTGATATTCCGACCAAGCCGTATGATGTCATTTTTGGCGAGGTTGGCCTGACCGGCGAA
GTTCGTGCCGTTTCAAGAGCAGAACAAAGGGTAAGAGAAGCGGAGAAGCTGGGTTTCAAGCGCGTACTCATGCCTGAGAA
AAGTTTGAAGGGATGGAAGCATCCCAAAGGAATACAAATCATAGGAGTAAACACTGTTGCAGATGCATTAGCGGTTGCGT
TAGATTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)

Subunit ChlI of Mg-chelatase (ChlI)

(346-427)

AAA domain (AAA_25)

(70-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A163HTJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.885

100

0.675
  radA Streptococcus pneumoniae Rx1

59.111

98.901

0.585
  radA Streptococcus pneumoniae D39

59.111

98.901

0.585
  radA Streptococcus pneumoniae R6

59.111

98.901

0.585
  radA Streptococcus pneumoniae TIGR4

59.111

98.901

0.585
  radA Streptococcus mitis NCTC 12261

59.111

98.901

0.585
  radA Streptococcus mitis SK321

58.889

98.901

0.582

Multiple sequence alignment