Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B7R82_RS02915 Genome accession   NZ_CP020858
Coordinates   593050..594423 (+) Length   457 a.a.
NCBI ID   WP_003702635.1    Uniprot ID   F5VC94
Organism   Ligilactobacillus salivarius strain ZLS006     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 588050..599423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7R82_RS02885 (B7R82_02880) - 588266..589210 (+) 945 WP_003700695.1 AEC family transporter -
  B7R82_RS02890 (B7R82_02885) - 589232..589906 (+) 675 WP_003706506.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
  B7R82_RS02895 (B7R82_02890) - 590076..590435 (-) 360 WP_003702606.1 bacteriocin immunity protein -
  B7R82_RS02900 (B7R82_02895) - 590639..591973 (-) 1335 WP_003700692.1 aminopeptidase C -
  B7R82_RS02905 (B7R82_02900) - 592062..592340 (-) 279 WP_003702618.1 GNAT family N-acetyltransferase -
  B7R82_RS02910 (B7R82_02905) - 592496..593032 (+) 537 WP_034983218.1 hypothetical protein -
  B7R82_RS02915 (B7R82_02910) radA 593050..594423 (+) 1374 WP_003702635.1 DNA repair protein RadA Machinery gene
  B7R82_RS02920 (B7R82_02915) - 594449..595561 (+) 1113 WP_087448630.1 PIN/TRAM domain-containing protein -
  B7R82_RS02925 (B7R82_02920) gltX 595692..597179 (+) 1488 WP_034983217.1 glutamate--tRNA ligase -
  B7R82_RS02930 (B7R82_02925) cysS 597331..598743 (+) 1413 WP_003702615.1 cysteine--tRNA ligase -
  B7R82_RS02935 (B7R82_02930) - 598736..599143 (+) 408 WP_003700685.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49722.37 Da        Isoelectric Point: 8.0254

>NTDB_id=226541 B7R82_RS02915 WP_003702635.1 593050..594423(+) (radA) [Ligilactobacillus salivarius strain ZLS006]
MAKTKTRYVCQNCEYISPRYLGRCPNCGSWNSLVEEIEKKESSTKAQPRVSISGTTVKPKLIDDVSTVETPRFKTNLEEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSTLGKKVLYVSGEESATQIKLRADRLGVHGTELYLYPETDMDSIR
KNIELLKPDFVVIDSVQTMQEPEMTAAVGSVSQIREVTGDLMQIAKTNGITVFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDQHRTFRILRAVKNRFGSTNEIGIFEMKNGGLSEVANPSEIFLEERLKGATGSAVVASLEGTRPILVELQALVTPTAF
GNAKRTTTGLDHNRVALLMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSLVSSYRDAETRPTDCFIGEVGLT
GEIRRVNRIEQRVVEAAKLGFKRVFLPKNNLDGWMPPKEIEVVGVSTLNQALKLIFS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=226541 B7R82_RS02915 WP_003702635.1 593050..594423(+) (radA) [Ligilactobacillus salivarius strain ZLS006]
TTGGCGAAAACTAAGACAAGATATGTTTGTCAAAATTGTGAATATATCTCTCCACGTTATTTAGGACGCTGCCCTAATTG
TGGTTCTTGGAATAGCTTGGTAGAGGAAATAGAGAAAAAGGAAAGTTCAACTAAAGCACAGCCTAGAGTTAGCATCTCTG
GTACAACAGTGAAACCTAAATTAATTGATGATGTTTCTACTGTGGAGACTCCAAGATTTAAAACTAATTTAGAAGAATTG
AATCGTGTTTTAGGTGGCGGAATTGTTCCCGGATCTTTAGTGTTAATTGGTGGAGATCCAGGTATTGGCAAGTCTACATT
ATTACTACAAGTTTCTGGACAATTATCTACTCTCGGGAAAAAGGTTCTTTATGTTTCTGGGGAAGAAAGTGCAACTCAAA
TTAAATTACGTGCGGATCGTTTAGGAGTTCATGGAACTGAGCTATATTTATATCCTGAAACAGATATGGATAGTATTAGA
AAAAATATTGAACTTTTAAAGCCTGATTTTGTTGTAATCGATTCGGTGCAGACAATGCAGGAGCCAGAAATGACAGCCGC
AGTTGGAAGTGTTTCTCAAATTAGGGAAGTAACTGGAGATTTGATGCAAATTGCGAAAACTAATGGGATAACTGTCTTTG
TTGTAGGACATGTTACTAAAGGTGGAGCAATTGCAGGGCCTAAAATTTTAGAACACATGGTAGATACTGTTCTTTATTTT
GAGGGAGATCAACATAGAACATTTAGAATTTTACGAGCTGTTAAAAATAGATTTGGTTCAACTAATGAAATTGGAATCTT
TGAAATGAAAAATGGAGGATTAAGCGAAGTTGCTAATCCATCAGAGATTTTTCTAGAAGAAAGATTAAAAGGGGCTACGG
GATCAGCTGTCGTTGCTTCTCTTGAAGGAACTAGACCCATATTGGTAGAATTACAAGCACTAGTAACACCGACTGCATTT
GGAAATGCTAAGCGAACAACAACTGGTTTAGATCACAATCGAGTTGCGTTACTGATGGCAGTACTTGAAAAAAGAGCTGG
TTTGTTATTGCAAAATCAGGATGCTTATTTGAAGGCTGCTGGTGGAGTGAAGTTAGATGAGCCAGCAATAGATTTAGCCA
TTGCTGTTAGTTTAGTTTCTAGTTATCGCGATGCAGAAACAAGACCAACTGACTGTTTTATAGGGGAAGTGGGATTAACT
GGTGAAATAAGACGAGTTAATCGTATTGAACAGCGTGTTGTTGAGGCTGCTAAGCTAGGATTTAAGCGAGTATTTTTACC
AAAGAACAACTTAGATGGTTGGATGCCTCCAAAAGAGATTGAAGTAGTAGGCGTAAGTACACTAAATCAAGCATTAAAAC
TAATTTTTAGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5VC94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.49

100

0.685
  radA Streptococcus mitis SK321

68.271

100

0.683
  radA Streptococcus pneumoniae Rx1

68.271

100

0.683
  radA Streptococcus pneumoniae D39

68.271

100

0.683
  radA Streptococcus pneumoniae R6

68.271

100

0.683
  radA Streptococcus pneumoniae TIGR4

68.271

100

0.683
  radA Bacillus subtilis subsp. subtilis str. 168

65.563

99.125

0.65

Multiple sequence alignment