Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C2R07_RS16445 Genome accession   NZ_CP026355
Coordinates   3169828..3170487 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain 3A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3164828..3175487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2R07_RS16415 ygiS 3166111..3167718 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  C2R07_RS16425 mqsA 3167851..3168246 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  C2R07_RS16430 mqsR 3168248..3168544 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  C2R07_RS16435 ygiV 3168749..3169231 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  C2R07_RS16440 ygiW 3169284..3169676 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C2R07_RS16445 ciaR 3169828..3170487 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  C2R07_RS16450 qseC 3170484..3171833 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  C2R07_RS16455 ygiZ 3171879..3172211 (-) 333 WP_000917684.1 DUF2645 family protein -
  C2R07_RS16465 mdaB 3172530..3173111 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C2R07_RS16470 ygiN 3173142..3173456 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C2R07_RS16475 parE 3173504..3175396 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=226541 C2R07_RS16445 WP_001221495.1 3169828..3170487(+) (ciaR) [Escherichia coli strain 3A]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=226541 C2R07_RS16445 WP_001221495.1 3169828..3170487(+) (ciaR) [Escherichia coli strain 3A]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361

Multiple sequence alignment