Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B5P37_RS07045 Genome accession   NZ_CP020773
Coordinates   1535322..1536689 (+) Length   455 a.a.
NCBI ID   WP_085237548.1    Uniprot ID   A0AAC9WJ92
Organism   Staphylococcus lutrae strain ATCC 700373     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1530322..1541689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5P37_RS07025 (B5P37_07025) - 1530373..1530834 (+) 462 WP_085237544.1 CtsR family transcriptional regulator -
  B5P37_RS07030 (B5P37_07030) - 1530849..1531379 (+) 531 WP_085237545.1 UvrB/UvrC motif-containing protein -
  B5P37_RS07035 (B5P37_07035) - 1531369..1532373 (+) 1005 WP_085237546.1 protein arginine kinase -
  B5P37_RS07040 (B5P37_07040) - 1532389..1534848 (+) 2460 WP_085237547.1 ATP-dependent Clp protease ATP-binding subunit -
  B5P37_RS07045 (B5P37_07045) radA 1535322..1536689 (+) 1368 WP_085237548.1 DNA repair protein RadA Machinery gene
  B5P37_RS07050 (B5P37_07050) - 1536710..1537771 (+) 1062 WP_085237549.1 PIN/TRAM domain-containing protein -
  B5P37_RS07055 (B5P37_07055) ispD 1537772..1538455 (+) 684 WP_085237550.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B5P37_RS07060 (B5P37_07060) ispF 1538463..1538941 (+) 479 Protein_1343 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  B5P37_RS07065 (B5P37_07065) gltX 1539017..1540471 (+) 1455 WP_085237551.1 glutamate--tRNA ligase -
  B5P37_RS07070 (B5P37_07070) cysE 1540849..1541496 (+) 648 WP_169710794.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49903.41 Da        Isoelectric Point: 7.2488

>NTDB_id=225833 B5P37_RS07045 WP_085237548.1 1535322..1536689(+) (radA) [Staphylococcus lutrae strain ATCC 700373]
MAKKKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTTSPKHGVRSEKASTAKIQKLHDIKQELAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSQEKKVLYVTGEESLNQTKIRADRLDEDASQLNVFAETDLVVIHEA
VKQLEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVMNPSEMFLEERSTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLSIIVATASSFKDQAVDGLDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRAIIPKNNIGGWHFPKGIEVIGVTSVNEALKYALK

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=225833 B5P37_RS07045 WP_085237548.1 1535322..1536689(+) (radA) [Staphylococcus lutrae strain ATCC 700373]
GTGGCAAAAAAGAAAACGATATTTGAATGTACCGCTTGTGGTTATCAATCACCGAAATGGATGGGAAAATGTCCGAACTG
TGGGGCATGGAATTCAATGGAAGAATCTTTTGAACAAAAGACAACCAGTCCGAAACATGGGGTACGCTCAGAGAAAGCAT
CAACAGCAAAGATACAAAAATTGCATGACATCAAGCAAGAACTCGCACCACGGATTCAAACGAATAGCGGTGAATTGAAT
CGTGTGCTCGGTGGTGGCATCGTAGAAGGTTCTCTCGTCCTCATTGGTGGGGATCCAGGTATCGGAAAGTCAACTTTATT
ACTACAAATGTGTGCGGCGTTATCACAAGAGAAAAAAGTACTGTATGTCACAGGGGAAGAATCACTCAATCAAACTAAAA
TAAGAGCAGATCGATTGGATGAAGATGCAAGCCAATTGAATGTTTTTGCTGAGACGGATTTGGTGGTTATTCATGAAGCG
GTGAAGCAGTTAGAACCGGATTTGATTGTTGTGGACTCCATTCAAACCATCTATCATCCGGAAATTAGTTCTGCACCGGG
ATCTGTTTCGCAAGTCCGTGAAAGCACGCAAAGTCTGATGCATATTGCTAAACAAATGAACATTGCGACGTTTATTGTAG
GGCATGTGACAAAAGAAGGCCAGATTGCGGGGCCGCGTTTACTCGAGCACATGGTGGATACGGTACTGTATTTTGAAGGA
GATGAGCATCACGCATATCGTATTTTAAGAGCAGTTAAAAACCGCTTCGGCTCGACGAATGAGATGGGGATCTTTGAAAT
GAAACAATCGGGATTGAAAAGTGTAATGAATCCCTCAGAAATGTTTTTAGAAGAACGATCAACTAATGTTGCGGGTTCTA
CGATTGTTGCGACAATGGAAGGGACACGCCCGTTACTTATTGAAGTGCAAGCATTAGTGACACCGACAACGTTTAATAAT
CCGAGACGTATGGCGACGGGGATCGACCATAATCGCCTTAGCCTATTGATGGCGGTATTGGAGAAAAAAGAAGGCTATTT
ACTTCAACAACAAGACGCCTATATTAAAGTGGCTGGAGGCGTGAAGTTGTCCGAGCCGGCAGTGGATTTGAGTATCATTG
TTGCAACGGCGTCTAGTTTCAAAGATCAGGCAGTAGATGGTCTGGATTGCTTTATTGGTGAGGTAGGACTTACAGGTGAA
GTCAGACGTGTATCAAGAATAGAACAACGTGTGCAAGAAGCAGCAAAATTAGGTTTCAAACGTGCAATTATTCCTAAAAA
TAATATTGGAGGATGGCATTTTCCGAAAGGGATTGAAGTGATAGGCGTTACCTCAGTGAATGAAGCGTTAAAATACGCAT
TAAAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

AAA domain (AAA_25)

(71-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.009

100

0.662
  radA Streptococcus pneumoniae Rx1

60.089

99.121

0.596
  radA Streptococcus pneumoniae D39

60.089

99.121

0.596
  radA Streptococcus pneumoniae R6

60.089

99.121

0.596
  radA Streptococcus pneumoniae TIGR4

60.089

99.121

0.596
  radA Streptococcus mitis NCTC 12261

60.089

99.121

0.596
  radA Streptococcus mitis SK321

59.867

99.121

0.593

Multiple sequence alignment