Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HM131_RS00565 Genome accession   NZ_CP020772
Coordinates   85051..86430 (-) Length   459 a.a.
NCBI ID   WP_085026943.1    Uniprot ID   A0A1W5ZQ66
Organism   Halobacillus mangrovi strain KTB 131     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 80051..91430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HM131_RS00550 (HM131_00550) - 81419..82651 (-) 1233 WP_332308726.1 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  HM131_RS00555 (HM131_00555) - 82720..83823 (-) 1104 WP_085026939.1 PIN/TRAM domain-containing protein -
  HM131_RS00560 (HM131_00560) disA 83978..85051 (-) 1074 WP_085026941.1 DNA integrity scanning diadenylate cyclase DisA -
  HM131_RS00565 (HM131_00565) radA 85051..86430 (-) 1380 WP_085026943.1 DNA repair protein RadA Machinery gene
  HM131_RS00570 (HM131_00570) clpC 86601..89060 (-) 2460 WP_085026946.1 ATP-dependent protease ATP-binding subunit ClpC -
  HM131_RS00575 (HM131_00575) - 89079..90149 (-) 1071 WP_085026948.1 protein arginine kinase -
  HM131_RS00580 (HM131_00580) - 90149..90697 (-) 549 WP_085026950.1 UvrB/UvrC motif-containing protein -
  HM131_RS00585 (HM131_00585) - 90718..91182 (-) 465 WP_085026952.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50031.66 Da        Isoelectric Point: 7.7298

>NTDB_id=225770 HM131_RS00565 WP_085026943.1 85051..86430(-) (radA) [Halobacillus mangrovi strain KTB 131]
MAKRKTKFVCQECGYETPKWMGKCPGCHQWNTLVEEVSAASTSSRHVFQTSSTSVSSKPEKINAIKSQKEPRLPTDMPEF
NRVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSAQIANKDMPVLYMSGEESPRQTKLRAERLGISSDQLYVLSETNLQDVI
NQIEAIDPKFVVIDSIQTIFKEDVTSAPGSVSQVRECTSQLMRIAKSKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRSVKNRFGSTHEMGIFEMKEQGLKEVLNPSEIFLEERSQGAAGSIVVASMEGTRPVLVEIQALISPTAF
GNPRRMATGLDNSRVPLLMAVLEKRAGLLLQNQDAYVKVAGGVKLDEPAIDLAVAISIASSFRDQASNPDDVVVGEVGLT
GEIRRVARIEQRVQEAAKLGFKRVIIPKKNMDGWTPPTEIQVIGVSTVQEAMKVTLGDA

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=225770 HM131_RS00565 WP_085026943.1 85051..86430(-) (radA) [Halobacillus mangrovi strain KTB 131]
TTGGCAAAAAGAAAAACAAAGTTTGTTTGCCAGGAATGTGGCTATGAAACACCCAAATGGATGGGGAAGTGTCCAGGGTG
TCACCAATGGAATACATTAGTCGAGGAAGTCAGTGCGGCCAGCACGAGTTCACGGCACGTTTTCCAAACGAGTTCCACGT
CAGTGTCGAGCAAACCAGAAAAAATAAATGCAATTAAATCTCAGAAAGAACCGCGTTTACCTACGGATATGCCAGAATTT
AACCGTGTTCTTGGAGGCGGAATCGTCGCAGGCTCACTGGTTTTAATTGGCGGAGATCCGGGTATCGGAAAATCAACCTT
ACTCCTTCAAGTGTCTGCTCAAATTGCGAATAAGGACATGCCGGTCCTTTATATGTCTGGGGAGGAGTCTCCACGACAGA
CAAAGCTTAGGGCAGAACGTTTAGGTATATCATCGGATCAGCTATACGTGCTGTCCGAAACCAACTTGCAAGATGTCATT
AATCAAATCGAGGCGATCGATCCTAAATTTGTGGTAATCGACTCGATTCAAACGATATTTAAAGAGGACGTAACTTCCGC
GCCAGGGAGCGTATCGCAGGTGCGTGAGTGTACAAGTCAACTCATGAGAATTGCCAAAAGTAAAGGTATCCCGATTTTTA
TTGTGGGGCATGTTACTAAAGAGGGTTCGATCGCAGGACCGAGGTTGCTCGAACATATGGTAGATGCCGTACTCTATTTC
GAAGGAGAACGTCATCATACATTCAGAATTCTAAGAAGCGTGAAAAACCGATTTGGAAGTACCCATGAGATGGGCATTTT
TGAAATGAAAGAACAAGGGTTGAAAGAAGTCCTCAATCCTTCTGAAATATTTCTGGAAGAACGATCACAGGGAGCGGCTG
GGTCGATTGTCGTTGCTTCTATGGAAGGGACGAGGCCCGTGCTCGTCGAAATCCAGGCATTGATCTCTCCGACAGCCTTT
GGTAATCCGAGACGCATGGCGACTGGCCTTGATAACAGCCGAGTTCCACTTTTAATGGCTGTGTTGGAAAAACGAGCGGG
GCTGCTTTTACAAAACCAGGACGCTTATGTGAAAGTTGCAGGTGGAGTGAAGCTTGATGAACCCGCAATTGACCTTGCTG
TAGCGATCAGCATTGCTTCAAGTTTTAGAGATCAGGCCTCCAACCCAGACGATGTGGTCGTCGGTGAGGTGGGGCTGACT
GGCGAGATCAGACGGGTCGCACGTATTGAACAGCGTGTGCAAGAAGCTGCTAAACTTGGATTTAAACGAGTGATTATTCC
TAAGAAGAATATGGATGGCTGGACACCGCCTACTGAAATTCAAGTCATCGGGGTTAGCACTGTACAAGAGGCTATGAAGG
TGACACTGGGGGATGCATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W5ZQ66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.21

99.564

0.719
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.797

98.693

0.63
  radA Streptococcus pneumoniae Rx1

63.576

98.693

0.627
  radA Streptococcus pneumoniae D39

63.576

98.693

0.627
  radA Streptococcus pneumoniae R6

63.576

98.693

0.627
  radA Streptococcus pneumoniae TIGR4

63.576

98.693

0.627

Multiple sequence alignment