Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BH695_RS00925 Genome accession   NZ_CP020771
Coordinates   179191..181206 (-) Length   671 a.a.
NCBI ID   WP_002742599.1    Uniprot ID   -
Organism   Microcystis aeruginosa PCC 7806SL     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 174191..186206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BH695_RS00915 (BH695_0194) - 176577..177791 (-) 1215 WP_036400185.1 type II secretion system F family protein -
  BH695_RS00920 (BH695_0195) pilT 177932..179038 (-) 1107 WP_002742598.1 type IV pilus twitching motility protein PilT Machinery gene
  BH695_RS00925 (BH695_0196) pilB/pilB1 179191..181206 (-) 2016 WP_002742599.1 GspE/PulE family protein Machinery gene
  BH695_RS00930 (BH695_0197) grpE 181447..182169 (+) 723 WP_002742600.1 nucleotide exchange factor GrpE -
  BH695_RS00935 (BH695_0198) - 182264..182905 (-) 642 WP_002742601.1 HAD-IA family hydrolase -
  BH695_RS00940 (BH695_0199) - 182996..184960 (-) 1965 WP_002742602.1 amylo-alpha-1,6-glucosidase -
  BH695_RS00945 (BH695_0200) rpmH 185052..185189 (+) 138 WP_002739276.1 50S ribosomal protein L34 -
  BH695_RS00950 (BH695_0201) rnpA 185216..185578 (+) 363 WP_084989793.1 ribonuclease P protein component -
  BH695_RS00955 (BH695_0202) - 185568..185963 (+) 396 WP_002738951.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75292.54 Da        Isoelectric Point: 6.5258

>NTDB_id=225755 BH695_RS00925 WP_002742599.1 179191..181206(-) (pilB/pilB1) [Microcystis aeruginosa PCC 7806SL]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPTAILIAMVDPDNLAATDDINRILRPRELGLQRLV
IAGEDYERLLEKFYQAQSELEREKARLQKEKELEKLSDLTDIVGSLDLTSGVANDEVADDLGEGDANQAPVINLVNKIIA
KALQEGSSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKIAPAVTARFKIMADLDISERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDRNTLQIFRDLASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGININTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTVIEAALTGHLVLTTLHTNDAAGAIVRLD
KMGIEPFMISGSLLGILAQRLVRRVCTQCRVAYHPSQEELARFGLSAANEQEVTFYKANTLTMEEIQQARKQGNLCLKCA
GSGYKDRIGVYEVMQNSERIQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTLTDSGLEAELKAK
RKSGLICSGCRAELQPEWLDCPYCMRPRFSY

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=225755 BH695_RS00925 WP_002742599.1 179191..181206(-) (pilB/pilB1) [Microcystis aeruginosa PCC 7806SL]
ATGACATTTTCTTCCAAGAAAACCCGTGCTGTCGCCCTACGCAATTACTTTTCCCCTTTTGGTAACAAGTTAGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAGCAGGCCCTGGTGGAAACGCGGAAATCGGGACGATCGCTAGTAGAAATATTGC
AAAAACTAACCGGGCGGCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAGTTGAGCGACGTGGATGGACTGGAAATAGCCAGGCTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGTCGAATCGAAGGAGAGCCAACCGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGCGGCTACTGACGATATCAATCGTATTCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTGCCGGGGAAGATTATGAAAGATTACTGGAAAAATTCTACCAAGCCCAATCGGAACTAGAACGGGAGAAAGCCCGACT
ACAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTGACCGATATTGTCGGCAGTCTTGATTTGACCTCTGGTGTTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGATGCTAATCAAGCCCCCGTCATCAACCTTGTCAATAAAATTATAGCT
AAAGCTCTACAGGAAGGATCCTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATCGCCCCAGCGGTCACAGCCCGGTTTAAAATCATGGCCGATC
TCGATATTTCCGAACGTCGTCTTCCCCAGGATGGTAAAATTCGGCGGATGTATCAAGGACGAAAAGTAGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTAAGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACCGATCGAAATACTCTGCAAATTTTCCGGGACTTGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CCACTGGTTCGGGAAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCCGGAATTAATATTAATACCGCC
GAAGACCCGATCGAATATTCTCTGCCAGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCCTGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGATAAGGAAACGGCTAAAACGGTGA
TCGAGGCCGCCTTAACCGGTCACTTGGTCTTAACTACCCTACACACTAACGATGCCGCAGGTGCGATCGTTCGTCTTGAT
AAAATGGGGATTGAACCATTTATGATTTCTGGTTCTCTGTTGGGAATCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAC
CCAGTGTCGGGTCGCCTATCACCCTAGTCAAGAGGAATTAGCCCGTTTTGGTCTTTCCGCTGCCAATGAACAGGAAGTGA
CTTTTTATAAGGCTAATACCCTAACTATGGAGGAAATTCAACAAGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTGCT
GGCAGCGGTTACAAAGATCGCATCGGTGTCTATGAGGTAATGCAGAATAGTGAACGAATACAGCAGTTAATTAACCAAGG
GGCGACAACCGATCGCATTAAGGAGGCGGCCGTTGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTAGAACGGGTGACACTGACCGATTCTGGTCTAGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCTGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGAGACC
GCGATTTAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.508

98.659

0.715

Multiple sequence alignment