Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   B7492_RS27895 Genome accession   NZ_CP020743
Coordinates   5274775..5276124 (-) Length   449 a.a.
NCBI ID   WP_157404932.1    Uniprot ID   -
Organism   Bacillus mycoides strain Gnyt1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5269775..5281124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7492_RS27870 (B7492_27840) - 5271316..5272254 (-) 939 WP_085312788.1 YafY family protein -
  B7492_RS27875 (B7492_27845) - 5272337..5272780 (+) 444 WP_002139133.1 VOC family protein -
  B7492_RS27880 (B7492_27850) hpf 5272884..5273426 (-) 543 WP_002089603.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  B7492_RS27885 (B7492_27855) cspC 5273747..5273944 (-) 198 WP_001990088.1 cold shock protein CspC -
  B7492_RS27890 (B7492_27860) - 5274071..5274775 (-) 705 WP_085312789.1 ComF family protein -
  B7492_RS27895 (B7492_27865) comFA 5274775..5276124 (-) 1350 WP_157404932.1 ATP-dependent helicase ComFA Machinery gene
  B7492_RS27900 (B7492_27870) - 5276255..5277568 (-) 1314 WP_085312791.1 NlpC/P60 family protein -
  B7492_RS27905 (B7492_27875) - 5277834..5278148 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  B7492_RS27910 (B7492_27880) - 5278310..5279152 (-) 843 WP_002016143.1 DegV family protein -
  B7492_RS27915 (B7492_27885) - 5279533..5280168 (+) 636 WP_002034752.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50884.26 Da        Isoelectric Point: 9.4468

>NTDB_id=225702 B7492_RS27895 WP_157404932.1 5274775..5276124(-) (comFA) [Bacillus mycoides strain Gnyt1]
MLAGRQLLLEELSSDLQDKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRIFASLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGILSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGVIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTIKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=225702 B7492_RS27895 WP_157404932.1 5274775..5276124(-) (comFA) [Bacillus mycoides strain Gnyt1]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGGATAAATTGGATCATTTGAAAAGGAATCG
AGACGTCGTTTGTGTACAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAA
TATTTGCATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCATTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGGGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTGTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTATTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTCATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.113

98.218

0.492
  comFA Latilactobacillus sakei subsp. sakei 23K

40.695

89.755

0.365

Multiple sequence alignment