Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   B7492_RS23585 Genome accession   NZ_CP020743
Coordinates   4503567..4505888 (-) Length   773 a.a.
NCBI ID   WP_002138444.1    Uniprot ID   A0A1W6ADW4
Organism   Bacillus mycoides strain Gnyt1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4498567..4510888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7492_RS35275 - 4499793..4499969 (-) 177 WP_002015300.1 hypothetical protein -
  B7492_RS23570 (B7492_23550) - 4500136..4500576 (-) 441 WP_002111994.1 D-tyrosyl-tRNA(Tyr) deacylase -
  B7492_RS23575 (B7492_23555) relA 4500594..4502777 (-) 2184 WP_085312421.1 GTP diphosphokinase -
  B7492_RS23580 (B7492_23560) - 4502989..4503501 (-) 513 WP_002015305.1 adenine phosphoribosyltransferase -
  B7492_RS23585 (B7492_23565) recJ 4503567..4505888 (-) 2322 WP_002138444.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  B7492_RS23590 (B7492_23570) - 4505990..4506883 (-) 894 WP_002015308.1 cation diffusion facilitator family transporter -
  B7492_RS23595 (B7492_23575) secDF 4507191..4509455 (-) 2265 WP_085312422.1 protein translocase subunit SecDF -
  B7492_RS23600 (B7492_23580) - 4509737..4510021 (-) 285 WP_002143227.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87266.65 Da        Isoelectric Point: 4.9265

>NTDB_id=225695 B7492_RS23585 WP_002138444.1 4503567..4505888(-) (recJ) [Bacillus mycoides strain Gnyt1]
MLQPKTRWKEKEYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVDFLNTADQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSLAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADVILTAEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSEEVLNKFSLEGYKEQLVHASEVAHFDDQYIVLLDLPKGTDELGD
VFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRDHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=225695 B7492_RS23585 WP_002138444.1 4503567..4505888(-) (recJ) [Bacillus mycoides strain Gnyt1]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTTGGCAGAGGCTTAAATACAGAAGATAAGATTGTAGACTTTTTAAATACAGCAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAAGCTATTGAAAATGGA
GAACAAATATTAATATTTGGCGATTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAGTTTTATATTCCAAATCGTTTTACTGAAGGCTATGGGCCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAATTACCAGAGGCACTTGCGATTATTCACCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGCCGTG
TGCCAGAGCATTTGTTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCACGGTGAAAATAGATTATTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGAAATATCGGTTTAAAGGCGTTATTTAAAGTCGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGATTCTCACTCGCACCTCGTATTAATGCGGTTGGTCGTTTGGAAGATGCAACAC
CTGCTGTGCACCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACTGAAGAAGCTATCGCTGAAGTCGAAAATAATTTCCCGCCAGAAGAAAATAAAGT
ATTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCAGCGGGAATGACGCTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGTAATTTTAACAGCAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAAGTAGAGGATGTAACGCTTGCCGCGATTGAGGATATGCAAAAGTTAGCGCCATTTGGCGTA
GGAAATCCGAAACCGCGTATTGCGGTGAAAGATGCCGACTTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGACACGATTGGATTTGGATTTGGTACTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGTGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGGCTATAAAGAACAGCTCGTGC
ATGCATCAGAGGTAGCTCATTTTGATGATCAATACATTGTGCTACTTGATTTGCCGAAAGGAACAGATGAATTAGGTGAT
GTATTTAAGGCTGGATTCCCAGCTCGAATTTACACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACGGTCCCAAC
GAGGGAACATTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCAGATAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAGAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTGATCATAAAT
AA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6ADW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.645

100

0.528

Multiple sequence alignment