Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   B7492_RS22780 Genome accession   NZ_CP020743
Coordinates   4348213..4349256 (-) Length   347 a.a.
NCBI ID   WP_085312372.1    Uniprot ID   A0A1W6AD15
Organism   Bacillus mycoides strain Gnyt1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4343213..4354256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7492_RS22745 (B7492_22725) - 4343263..4343871 (+) 609 WP_085312368.1 hypothetical protein -
  B7492_RS35260 - 4344210..4344362 (-) 153 WP_088068512.1 hypothetical protein -
  B7492_RS22750 (B7492_22730) - 4344663..4345832 (+) 1170 WP_085312369.1 site-specific integrase -
  B7492_RS22755 (B7492_22735) - 4345959..4346153 (-) 195 Protein_4453 Tfp pilus assembly protein FimT/FimU -
  B7492_RS22760 (B7492_22740) - 4346137..4346433 (-) 297 WP_016100615.1 hypothetical protein -
  B7492_RS22765 (B7492_22745) comGD 4346426..4346881 (-) 456 WP_085312371.1 competence type IV pilus minor pilin ComGD -
  B7492_RS22770 (B7492_22750) comGC 4346878..4347177 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  B7492_RS22775 (B7492_22755) comGB 4347189..4348226 (-) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  B7492_RS22780 (B7492_22760) comGA 4348213..4349256 (-) 1044 WP_085312372.1 competence type IV pilus ATPase ComGA Machinery gene
  B7492_RS22785 (B7492_22765) - 4349462..4350157 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  B7492_RS22790 (B7492_22770) - 4350439..4350681 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  B7492_RS22795 (B7492_22775) - 4350789..4352183 (+) 1395 WP_002138338.1 L-cystine transporter -
  B7492_RS22800 (B7492_22780) - 4352264..4352656 (-) 393 WP_085312373.1 hypothetical protein -
  B7492_RS22805 (B7492_22785) - 4352744..4352959 (-) 216 WP_002015115.1 DUF3912 family protein -
  B7492_RS22810 (B7492_22790) - 4353212..4353523 (+) 312 WP_002165070.1 hypothetical protein -
  B7492_RS22815 (B7492_22795) - 4353559..4354047 (-) 489 WP_085312374.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39278.76 Da        Isoelectric Point: 9.3368

>NTDB_id=225691 B7492_RS22780 WP_085312372.1 4348213..4349256(-) (comGA) [Bacillus mycoides strain Gnyt1]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=225691 B7492_RS22780 WP_085312372.1 4348213..4349256(-) (comGA) [Bacillus mycoides strain Gnyt1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGCTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6AD15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363

Multiple sequence alignment