Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   B7492_RS06615 Genome accession   NZ_CP020743
Coordinates   1259761..1261587 (+) Length   608 a.a.
NCBI ID   WP_085309743.1    Uniprot ID   A0A1W6A4Y2
Organism   Bacillus mycoides strain Gnyt1     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1254761..1266587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7492_RS06595 (B7492_06595) - 1254972..1255646 (-) 675 WP_002011349.1 TerC family protein -
  B7492_RS06600 (B7492_06600) mecA 1256083..1256766 (+) 684 WP_002011351.1 adaptor protein MecA Regulator
  B7492_RS06605 (B7492_06605) - 1256839..1258383 (+) 1545 WP_002064255.1 cardiolipin synthase -
  B7492_RS06610 (B7492_06610) - 1258475..1259710 (+) 1236 WP_044437788.1 competence protein CoiA -
  B7492_RS06615 (B7492_06615) pepF 1259761..1261587 (+) 1827 WP_085309743.1 oligoendopeptidase F Regulator
  B7492_RS34940 - 1261721..1261894 (-) 174 WP_000748141.1 hypothetical protein -
  B7492_RS06620 (B7492_06620) - 1262124..1263017 (-) 894 WP_085309745.1 ClpXP adapter SpxH family protein -
  B7492_RS06625 (B7492_06625) - 1263017..1263415 (-) 399 WP_000043388.1 hypothetical protein -
  B7492_RS06630 (B7492_06630) - 1263601..1264179 (-) 579 WP_085309747.1 CYTH domain-containing protein -
  B7492_RS06635 (B7492_06635) - 1264324..1264695 (+) 372 WP_002011362.1 hypothetical protein -
  B7492_RS06640 (B7492_06640) - 1264726..1265364 (+) 639 WP_002125951.1 GTP pyrophosphokinase family protein -
  B7492_RS06645 (B7492_06645) - 1265383..1266180 (+) 798 WP_002011365.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70361.72 Da        Isoelectric Point: 4.6480

>NTDB_id=225674 B7492_RS06615 WP_085309743.1 1259761..1261587(+) (pepF) [Bacillus mycoides strain Gnyt1]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLQENRELSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSTSSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVFETYGKFKNTFAST
LSGAVKRNNFNARIRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVQEVKM
NVKYEEAQDLLLKSLNVLGDEYVEILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLYTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDKEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=225674 B7492_RS06615 WP_085309743.1 1259761..1261587(+) (pepF) [Bacillus mycoides strain Gnyt1]
ATGTCTGAACAAAATAAAGCAAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGCACATGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTGCCAAAGTTAACTGAATTTAAAGGGA
AGCTTGGCGACTCTGCAGACAATTTGCTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCACTGAATGATCGCGCAACAAATTTATA
CTCACAAGTATCTAGTAGCACAGCGTATATCGTACCTGAAATTTTATCTATTTCAGAAGATACATTGCAAACATTCTTAC
AAGAAAATCGAGAACTAAGTGTATATGAGCATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCTGAGGCA
GAGGAAGCGTTATTAGCAGAAGCATCTGAAGTGATGAGTACATCAAGTAATACATTCGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAGGTAGAAATAACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTTCGCGAAGATGCATTCAAAGCTGTATTTGAAACGTATGGGAAATTTAAGAACACATTTGCAAGTACG
TTAAGCGGGGCAGTGAAACGTAATAATTTCAATGCACGTATTCGTAAATATGACTCTGCTCGCCAAGCAGCATTAAGCAA
TAATAATATTCCTGAAGCAGTATACGATCAACTCGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATTG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTACGATTTATATACACCACTTGTACAAGAAGTGAAAATG
AATGTAAAGTATGAAGAAGCGCAAGACCTGTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGAAATTTTAAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTACGAGAATAAAGGCAAACGAAGCGGAGCATATTCATCTGGTGCATACG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATATACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTATTACACAAGAAAGACACAGCCACACGTATACGGTGATTATTCAATCTTCGTTGCAGAGGTAGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAAAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACAATGTTTGCAGAGTTCGAGCATATCATTCATAAAAAAGTACAA
GAAGGACATGCGGTTACGCCAGATATGTTAACGGAGATCTACTACGATTTAAATAAGAAATATTTCGGGGATGCTTTAGT
AATCGATAAAGAAATCGGTTTAGAATGGTCTCGTATTCCGCACTTCTATTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCGTTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGTTACATTAACGAATTC
TTAAAAGCGGGAAGCTCCGATTATCCAATTGAAGTGTTGAAAAAAGCAGGAGTTGATATGGCATCTCCTGAACCAGTGAA
AGAGGCACTTCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCGTTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6A4Y2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.747

97.533

0.485

Multiple sequence alignment