Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   B7492_RS06290 Genome accession   NZ_CP020743
Coordinates   1202600..1206325 (+) Length   1241 a.a.
NCBI ID   WP_085309704.1    Uniprot ID   -
Organism   Bacillus mycoides strain Gnyt1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1197600..1211325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7492_RS06275 (B7492_06275) - 1197757..1198350 (+) 594 WP_002140801.1 TVP38/TMEM64 family protein -
  B7492_RS06280 (B7492_06280) lepB 1198407..1198970 (+) 564 WP_085309700.1 signal peptidase I -
  B7492_RS06285 (B7492_06285) addB 1199088..1202603 (+) 3516 WP_085309702.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  B7492_RS06290 (B7492_06290) addA 1202600..1206325 (+) 3726 WP_085309704.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  B7492_RS06295 (B7492_06295) - 1206349..1206627 (+) 279 WP_085309706.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  B7492_RS06300 (B7492_06300) - 1206723..1207430 (+) 708 WP_085309708.1 DNA alkylation repair protein -
  B7492_RS06305 (B7492_06305) gerPF 1207483..1207698 (-) 216 WP_001141570.1 spore germination protein GerPF -
  B7492_RS06310 (B7492_06310) - 1207741..1208127 (-) 387 WP_002125894.1 spore germination protein GerPE -
  B7492_RS06315 (B7492_06315) gerPD 1208142..1208336 (-) 195 WP_001052805.1 spore germination protein GerPD -
  B7492_RS06320 (B7492_06320) - 1208343..1208957 (-) 615 WP_085309710.1 spore germination protein GerPC -
  B7492_RS06325 (B7492_06325) gerPB 1209026..1209232 (-) 207 WP_001012506.1 spore germination protein GerPB -
  B7492_RS06330 (B7492_06330) gerPA 1209247..1209468 (-) 222 WP_001111190.1 spore germination protein GerPA -
  B7492_RS06335 (B7492_06335) - 1209565..1209744 (-) 180 WP_002087032.1 aspartyl-phosphate phosphatase Spo0E family protein -
  B7492_RS06340 (B7492_06340) - 1209988..1210890 (+) 903 WP_085309712.1 fumarylacetoacetate hydrolase family protein -
  B7492_RS06345 (B7492_06345) - 1210925..1211209 (-) 285 WP_000925332.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142539.81 Da        Isoelectric Point: 4.8990

>NTDB_id=225669 B7492_RS06290 WP_085309704.1 1202600..1206325(+) (addA) [Bacillus mycoides strain Gnyt1]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEKHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSGQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYSASWK
VEVVEGKDLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYADATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKYPVKEKCLYFFDGNHVVNIDE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=225669 B7492_RS06290 WP_085309704.1 1202600..1206325(+) (addA) [Bacillus mycoides strain Gnyt1]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCGGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCGCAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACAATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAACTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAACCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAAAAGCATATT
CGTAAGGCGACTGAACTCGCGATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACTTTACAAGCGGATTTAGCTTTACT
TGGAACGTTATCCTCAGCAGCGCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAAAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCCGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGGGCAAAGTGAAGACGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGTGAAGAAGTCGGCGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACTGAGGAAGAAGTGCAAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCGGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTCATTGATAAC
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAGGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGTCAACAATCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTGCGCTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCCCTCGGAGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACAGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATACGCGCTGGAGCGTCATGTTATTTAGATTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAGGTGAAATTTATGAATATAGTGCTAGCTGGAAA
GTAGAAGTTGTTGAAGGAAAAGATTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
TGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTGATGTGGGAATACGGGTATGCGG
ACGCGACATCTCACCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAACGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAAACAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAATATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGAAATCATGTTGTAAATATTGACGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.569

100

0.538

Multiple sequence alignment