Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B7492_RS00535 Genome accession   NZ_CP020743
Coordinates   97770..99146 (+) Length   458 a.a.
NCBI ID   WP_085308581.1    Uniprot ID   A0A1W6A212
Organism   Bacillus mycoides strain Gnyt1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92770..104146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7492_RS00515 (B7492_00515) ctsR 92969..93430 (+) 462 WP_002029353.1 transcriptional regulator CtsR -
  B7492_RS00520 (B7492_00520) - 93600..94148 (+) 549 WP_085308579.1 UvrB/UvrC motif-containing protein -
  B7492_RS00525 (B7492_00525) - 94153..95217 (+) 1065 WP_002139949.1 protein arginine kinase -
  B7492_RS00530 (B7492_00530) clpC 95240..97675 (+) 2436 WP_002063405.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  B7492_RS00535 (B7492_00535) radA 97770..99146 (+) 1377 WP_085308581.1 DNA repair protein RadA Machinery gene
  B7492_RS00540 (B7492_00540) disA 99150..100223 (+) 1074 WP_000392162.1 DNA integrity scanning diadenylate cyclase DisA -
  B7492_RS00545 (B7492_00545) - 100384..101487 (+) 1104 WP_002063406.1 PIN/TRAM domain-containing protein -
  B7492_RS00550 (B7492_00550) ispD 101504..102184 (+) 681 WP_085308583.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B7492_RS00555 (B7492_00555) ispF 102298..102774 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49862.54 Da        Isoelectric Point: 7.4250

>NTDB_id=225662 B7492_RS00535 WP_085308581.1 97770..99146(+) (radA) [Bacillus mycoides strain Gnyt1]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVTQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPADAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNFGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=225662 B7492_RS00535 WP_085308581.1 97770..99146(+) (radA) [Bacillus mycoides strain Gnyt1]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAATGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TCAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGAGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATTCAGACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGGAGTGTAACGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCTGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTTGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TCGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAGCCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGGAAAAATTTTGGGGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6A212

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629

Multiple sequence alignment