Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   C2R19_RS18115 Genome accession   NZ_CP026344
Coordinates   3465543..3466220 (+) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain 8FA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3460543..3471220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2R19_RS18080 gspG 3461023..3461460 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  C2R19_RS18085 gspH 3461468..3461977 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  C2R19_RS18090 gspI 3461974..3462351 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  C2R19_RS18095 gspJ 3462344..3462931 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  C2R19_RS18100 gspK 3462924..3463907 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  C2R19_RS18105 gspL 3463922..3465085 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  C2R19_RS18110 gspM 3465082..3465543 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  C2R19_RS18115 pilD 3465543..3466220 (+) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  C2R19_RS18120 bfr 3466249..3466725 (-) 477 WP_000675504.1 bacterioferritin -
  C2R19_RS18125 bfd 3466797..3466991 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  C2R19_RS18130 chiA 3467160..3469853 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=225644 C2R19_RS18115 WP_000178154.1 3465543..3466220(+) (pilD) [Escherichia coli strain 8FA]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=225644 C2R19_RS18115 WP_000178154.1 3465543..3466220(+) (pilD) [Escherichia coli strain 8FA]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment