Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   B7P44_RS15345 Genome accession   NZ_CP020737
Coordinates   3268289..3269794 (+) Length   501 a.a.
NCBI ID   WP_084905547.1    Uniprot ID   A0A2A4FFX3
Organism   Burkholderia ubonensis subsp. mesacidophila strain ATCC 31433     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3263289..3274794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7P44_RS15320 kdsD 3264527..3265510 (+) 984 WP_084905538.1 arabinose 5-phosphate isomerase KdsD -
  B7P44_RS15325 - 3265510..3266046 (+) 537 WP_084905540.1 HAD family hydrolase -
  B7P44_RS15330 lptC 3266050..3266664 (+) 615 WP_084905542.1 LPS export ABC transporter periplasmic protein LptC -
  B7P44_RS15335 lptA 3266685..3267350 (+) 666 WP_084905543.1 lipopolysaccharide transport periplasmic protein LptA -
  B7P44_RS15340 lptB 3267347..3268123 (+) 777 WP_084905545.1 LPS export ABC transporter ATP-binding protein -
  B7P44_RS15345 rpoN/rpoN1 3268289..3269794 (+) 1506 WP_084905547.1 RNA polymerase factor sigma-54 Machinery gene
  B7P44_RS15350 hpf 3269953..3270321 (+) 369 WP_017334258.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  B7P44_RS15355 - 3270513..3271025 (+) 513 WP_084905549.1 PTS sugar transporter subunit IIA -
  B7P44_RS15360 hprK 3271157..3272125 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  B7P44_RS15365 rapZ 3272202..3273110 (+) 909 WP_084905551.1 RNase adapter RapZ -
  B7P44_RS15370 - 3273137..3273775 (+) 639 WP_084905553.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54823.11 Da        Isoelectric Point: 4.8190

>NTDB_id=225608 B7P44_RS15345 WP_084905547.1 3268289..3269794(+) (rpoN/rpoN1) [Burkholderia ubonensis subsp. mesacidophila strain ATCC 31433]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNPEPMA
ATNGAGQTGERTERDEPAATDDYDGYAAGDSDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=225608 B7P44_RS15345 WP_084905547.1 3268289..3269794(+) (rpoN/rpoN1) [Burkholderia ubonensis subsp. mesacidophila strain ATCC 31433]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACTTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCAGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCGTCGAACCCCGAGCCGATGGCG
GCCACGAACGGCGCTGGCCAGACCGGCGAGCGCACCGAACGCGACGAGCCGGCCGCCACGGACGACTACGACGGCTACGC
CGCCGGCGACAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGACGACGACGACCTGC
CGCCGCTGCAGGTCCAGGAGGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGGGTCACGCAGGCCGGGCCG
CGCGACCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCAACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGACTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGTATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGCTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CGGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCTGAGCTGAATCCCGAGGTCATGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
ACTGCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGGTTCGACACGATCCTGCGTGTTGCACAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACCCAAAATCGCCTCTTTCGGACAGCCGAATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691

Multiple sequence alignment